Nucleotide substitution: G?A. Contain a 5'-splice junction which has been altered from GTttGT to GTttaT. Also contains a 9nt deletion that changes the prredicted peptide sequence in a putative membrane-spanning segment from SDTAVTLLFTAE to SDTVLFTAE.
The average amount of daily sleep in nahar38 flies is comparable to that of wild type.
The EC[[50]] value for the volatile anesthetics isoflurane and sevoflurane are significantly decreased in the mutant flies compared to wild type.
Mutants are noticeably smaller than wild-type but not obviously sick or deformed. The majority of homozygous mutants that have been entrained to diurnal lighting (LD, 12hr light:12ht dark) and subsequently monitored under constant darkness (DD) are arrhythmic - only a small number are strongly rhythmic. In contrast to wild-type however, under LD entrainment however mutants exhibit most activity at night.
nahar38 mutant flies are hypersensitive to both halothane and enflurane when tested in both regular and red light.
Mutant flies are resistant to halothane in an inebriometer assay compared to control flies, having a lower Response Index of 0.24 +/- 0.05 after 30 minutes of exposure to halothane (compared to 0.90 +/- 0.03 for control flies).
Mutants show almost complete resistance to halothane, withstanding 30 minute exposure to halothane in an inebriometer, and a characteristic saccadic walking behaviour in the absence of anesthetics. Shows appreciable resistance to trichloroethylene-induced anesthesia. Adult abdomens are narrow and more cylindrical than wild type. Response to enflurane and isoflurane is indistinguishable from wild type. Mosaic analysis reveals that the focus for anesthetic resistance maps close to the focus for the geotaxis defect, in the head, mapping back to the cephalic region in the blastoderm.
Homozygotes show resistance to halothane in an inebriometer assay (an assay of geotactic and postural behaviour) compared to wild-type flies.
Individuals are able to remain standing upright in the presense of halothane.
In the absence of anaesthetics flies show several mutant phenotypes. Fewer offspring are produced compared to wild type. Abdomens are more slender and elongated than wild type. Flies show hesitancy when walking, mechanical shock enhances the hesitancy. When exposed to anaesthetics individuals become immobile, they freeze, and then fall over.
nahar38 has chemical sensitive phenotype, enhanceable by w1118
nahar38, w1118 mutant flies show the same sensitivity to 0.1% enflurane or halothane when tested under red light as nahar38 mutant flies do. However, nahar38, w1118 mutant flies show a much higher sensitivity to 0.05% enflurane or halothane when tested under ambient light than nahar38 mutant flies do.
Alleles of na form a phenotypic series, going from strongest to weakest: nahar38 > nahar85 > naP890 > na1 > naPex1 > naPex2.