Alteration in the 5' splice donor site. The consensus 5' splice donor GT is changed to AT. This would potentially allow an upstream cryptic splice donor to be used, thus changing the N-terminal amino acids of the encoded protein.
G2724386A
Alteration in 5' splice donor site
Eggs laid by homozygous mutant mothers exhibit three classes of phenotype. Pronuclear arrest embryos exhibit pronuclear arrest at the end of meiosis. Type II embryos escape pronuclear arrest and undergo a few rounds of nuclear division before arresting. Type I embryos degenerate from pronuclear or Type II arrests.
Females doubly mutant for gnu305 and fs(1)Ya5 or fs(1)Ya14-77 exhibit gnu-like or fs(1)Ya-like nuclear morphology.
Mutant embryos arrest either with multiple abnormal nuclei or at the pronuclear stage, with fewer than four abnormally condensed nuclei, presumably derived from the sperm and female meiotic products. 67% of embryos are pronuclear arrested, 33% have multiple abnormal nuclei. Type II embryos of the multiple nuclear arrested class show fewer than 16 nuclei, which are not fragmented. These embryos never develop to the blastoderm stage.
Weak allele. Most embryos from females homozygous for fs(1)Ya14-77 arrest at multinuclear stages rather than at the pronuclear fusion stage (in contrast to the strong fs(1)Ya2 allele). More than 10% of these multinuclear embryos arrest as gastrulae.
Eggs are laid.
Homozygous females lay eggs that fail to hatch.
fs(1)Ya14-77 has embryo phenotype, suppressible by RpLP0306
The addition of RpLP0306 to homozygous fs(1)Ya14-77 mothers, reduces the number of embryos laid with the pronuclear arrest phenotype.
fs(1)Ya14-77 is rescued by fs(1)YaFAPKK.Hsp26
fs(1)Ya14-77 is rescued by fs(1)YaΔQ.ΔS:T.Hsp26
fs(1)Ya14-77 is rescued by fs(1)YaSY160.Hsp26
fs(1)Ya14-77 is rescued by fs(1)YaFEPKK.Hsp26
fs(1)Ya14-77 is rescued by fs(1)YaΔS:T.Hsp26
fs(1)Ya14-77 is rescued by fs(1)YaN1.Hsp26.Tag:HA
fs(1)Ya14-77 is rescued by fs(1)YaHsp26.PL
fs(1)Ya14-77 is rescued by fs(1)YaΔQ.Hsp26
fs(1)Ya14-77 is rescued by fs(1)YaΔFSPKK.Hsp26
fs(1)Ya14-77 is partially rescued by fs(1)YaN3.Hsp26.Tag:HA
fs(1)Ya14-77 is partially rescued by fs(1)YaC3.Hsp26.Tag:HA
fs(1)Ya14-77 is partially rescued by fs(1)YaΔITPIR.ΔFSPKK.Hsp26
fs(1)Ya14-77 is partially rescued by fs(1)YaIAPIR.FAPKK.Hsp26
fs(1)Ya14-77 is partially rescued by fs(1)YaIAPIR.Hsp26
fs(1)Ya14-77 is partially rescued by fs(1)YaΔITPIR.Hsp26
fs(1)Ya14-77 is not rescued by fs(1)YaC2.Hsp26
fs(1)Ya14-77 is not rescued by fs(1)YaC2.SY160.Hsp26
fs(1)Ya14-77 is not rescued by fs(1)YaC1.C2.Hsp26
fs(1)Ya14-77 is not rescued by fs(1)YaC2.FAPKK.Hsp26
fs(1)Ya14-77 is not rescued by fs(1)YaC1.C2.FAPKK.Hsp26
fs(1)Ya14-77 is not rescued by fs(1)YaC1.Hsp26
fs(1)Ya14-77 is fully rescued by fs(1)YaHsp26.PL, fs(1)YaN1.Hsp26.T:Ivir\HA1, fs(1)YaSY160.Hsp26, fs(1)YaΔQ.Hsp26, fs(1)YaΔS:T.Hsp26, fs(1)YaΔFSPKK.Hsp26, fs(1)YaFAPKK.Hsp26, fs(1)YaΔQ.ΔS:T.Hsp26 and fs(1)YaFEPKK.Hsp26 under both heat shock and non-heat shock conditions. fs(1)Ya14-77 is not rescued by fs(1)YaC1.Hsp26, fs(1)YaC2.Hsp26, fs(1)YaC1.C2.Hsp26, fs(1)YaC2.SY160.Hsp26, fs(1)YaC2.FAPKK.Hsp26, fs(1)YaC1.C2.FAPKK.Hsp26 or fs(1)Ya4.28.1.1 under either heat shock and non-heat shock conditions. fs(1)Ya14-77 is not rescued under non-heat shock conditions, and very weakly rescued under heat shock conditions by fs(1)YaN3.Hsp26.T:Ivir\HA1. fs(1)Ya14-77 is partially rescued by fs(1)YaΔITPIR.Hsp26, fs(1)YaIAPIR.Hsp26, fs(1)YaΔITPIR.ΔFSPKK.Hsp26 and fs(1)YaIAPIR.FAPKK.Hsp26 under both heat shock and non-heat shock conditions. fs(1)Ya14-77 is very weakly rescued under non-heat shock conditions, and partially rescued under heat shock conditions by fs(1)YaC3.Hsp26.T:Ivir\HA1.
Partially complements fs(1)Ya5 for female fertility: 15% of transheterozygotes develop to adulthood.
Leaky mutation.