FB2024_03 , released June 25, 2024
Allele: Dmel\PuZ22
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General Information
Symbol
Dmel\PuZ22
Species
D. melanogaster
Name
FlyBase ID
FBal0033971
Feature type
allele
Associated gene
Dmel\Pu
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        PuZ22 adult heterozygous males show significant decrease in locomotion activity compared to wild-type controls.

        (Wakabayashi-Ito et al., 2015)

        Homozygous embryos have a disrupted tracheal network, with misdirected tubules and ectopic branching in addition to disruption of the dorsal trunk.

        67% of heterozygous embryos show ectopic tracheal migration.

        (Hsouna et al., 2007)

        Cuticle defects.

        (Lindsley and Zimm, 1992)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Enhancer of
        Statement
        Reference

        Pu[+]/PuZ22 is an enhancer of partially lethal - majority die phenotype of TorsinKO13

        (Wakabayashi-Ito et al., 2011)
        Other
        Phenotype Manifest In
        Enhanced by
        Statement
        Reference

        PuZ22 has embryonic/larval tracheal system phenotype, enhanceable by awdj2A4/awd[+]

        (Hsouna et al., 2007)

        PuZ22 has embryonic/larval tracheal system phenotype, enhanceable by shi2

        (Hsouna et al., 2007)
        Suppressed by
        Statement
        Reference

        PuZ22 has embryonic/larval tracheal system phenotype, suppressible | partially by btlH82Δ3/btl[+]

        (Hsouna et al., 2007)

        PuZ22 has embryonic/larval tracheal system phenotype, suppressible | partially by Catsup[+]/Catsup26

        (Hsouna et al., 2007)
        Enhancer of
        Statement
        Reference

        Pu[+]/PuZ22 is an enhancer of embryonic/larval tracheal system phenotype of awdj2A4

        (Hsouna et al., 2007)

        Pu[+]/PuZ22 is an enhancer of embryonic/larval tracheal system phenotype of shi2

        (Hsouna et al., 2007)
        Suppressor of
        Statement
        Reference

        Pu[+]/PuZ22 is a suppressor of embryonic/larval tracheal system phenotype of Catsup26

        (Hsouna et al., 2007)

        Pu[+]/PuZ22 is a suppressor of embryonic/larval tracheal system phenotype of btlH82Δ3

        (Hsouna et al., 2007)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Double heterozygous TorsinKO13/+; PuZ22/+ female larvae show very significantly reduced peristaltic frequency relative to either single heterozygote.

        While TorsinKO13/Y third instar larvae are mostly viable, very few TorsinKO13/Y ; PuZ22/+ third instar larvae are observed.

        (Wakabayashi-Ito et al., 2011)

        The penetrance and severity of the ectopic tracheal migration phenotype is increased in PuZ22/+ awdj2A4 double mutant embryos compared to either single mutant.

        The penetrance and severity of the ectopic tracheal migration phenotype is increased in PuZ22/+ shi2 double mutant embryos compared to either single mutant.

        The opposing tracheal phenotypes of PuZ22/+ and btlH82Δ3/+ single heterozygous embryos (ectopic migration and lack of migration respectively) are both partially suppressed in PuZ22/+ btlH82Δ3/+ double heterozygous embryos.

        The ectopic tracheal migration phenotype of PuZ22/+ single heterozygous embryos is partially suppressed and the lack of tracheal migration phenotype of Catsup26/+ single heterozygous embryos is completely suppressed in PuZ22/+ Catsup26/+ double heterozygous embryos.

        (Hsouna et al., 2007)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Partially rescued by

        PuZ22 is partially rescued by PuUAS.cHa/Scer\GAL4btl.PS

        (Hsouna et al., 2007)
        Comments

        Expression of PuScer\UAS.cHa under the control of Scer\GAL4btl.PS can rescue the ectopic tracheal branch migration see in PuZ22 embryos.

        (Hsouna et al., 2007)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        Comments
        Comments

        Class II Pu allele.

        (Lindsley and Zimm, 1992)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        PuZ22
        (Wakabayashi-Ito et al., 2015, Wakabayashi-Ito et al., 2011, Hsouna et al., 2007)
        Name Synonyms
        Secondary FlyBase IDs
          References (4)