FB2024_03 , released June 25, 2024
Allele: Dmel\PTΔ2-3
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General Information
Symbol
Dmel\P\TΔ2-3
Species
D. melanogaster
Name
FlyBase ID
FBal0033895
Feature type
allele
Associated gene
P\T
Associated Insertion(s)
Carried in Construct
H{PΔ2-3}
H{w+.Δ2-3.M}
P{Δ2-3}
Also Known As
Δ2-3
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Lemaitre et al., 1993, Banga et al., 1991, Laski et al., 1986)
Progenitor genotype
P\T+tPryB
(Laski et al., 1986)
Carried in construct
H{PΔ2-3}
H{w+.Δ2-3.M}
P{Δ2-3}
Cytology
Description

The last intron, which is responsible for the limitation of transposase activity to the germline, has been deleted. P\TΔ2-3 therefore produces transposase in both the somatic and germ cells.

(Ronsseray et al., 1991)

P\TΔ2-3 lacks a tissue-specific intron that normally limits transposase activity to the germline.

(Engels et al., 1990)

Precise deletion of the intron sequences at the ORF2 and ORF3 junction.

(Laski et al., 1986)
Allele components
Component
Use(s)
Regulatory region(s)
P\T
Encoded product / tool
P\T
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The level of mutability of snw in the presence of P\TΔ2-3 is not reduced by the presence of P\TSP.hs or P\TTP6.hs.

      The level of mutability of snw in the presence of P\TΔ2-3 is significantly reduced by the presence of P\TKP.hs or P\TTP5.hs (although P\TTP5.hs is less effective than P\TKP.hs).

      (Jensen et al., 2008)

      P{KP}2F represses the transposase activity of P{Δ2-3}99B in the germline; the rate of snw mutation in the male germline caused by P{Δ2-3}99B is reduced if P{KP}2F is present. H{hsp/KP} represses the transposase activity of P{Δ2-3}99B in the germline; the rate of snw mutation in the male germline caused by P{Δ2-3}99B is reduced if H{hsp/KP} is present. P{KP}13F does not repress the transposase activity of P{Δ2-3}99B in the germline; the rate of snw mutation in the male germline caused by P{Δ2-3}99B is not affected if P{KP}13F is present.

      (Simmons et al., 2002)

      There is no detectable maternally transmited transposase activity due to P\TΔ2-3, as measured by the rate of both somatic and germ-line mutation of snw (a P-element insertion allele highly mutable by P\TΔ2-3).

      (Simmons et al., 2002)

      No repression of P-element mobility in somatic tissues.

      (Lemaitre et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (47)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Transposase activity is present in the germline and soma.

      (Laski and Rubin, 1989)

      P\TΔ2-3 produces transposase, but is not mobile.

      (Engels et al., 1990)

      Assayed for transposase activity in the germline using the snw assay. Assayed for transposase activity in the soma using the snw and P{wA}038 assays.

      (Chain et al., 1991)

      Distribution of male recombination events, in the absence of P element targets, approximates that seen in studies of radiation-induced crossing-over. Where there is a single P element target, P{lArB}A53.1M3, crossovers increased 58-fold in the immediate region of the P element target.

      (McCarron et al., 1994)

      99B insert consists of two copies of the original insert in head-to-head orientation with approximately 360bp missing from the junction of the 5' P-element ends (1-19 of one 5' end and 1-348 of the other). Stability of the insertion can be explained by the insert having two functional 3' ends (mobilisation of an insertion with symmetrical ends by transposase is less efficient) and the composite element is 20kb long again causing less efficient mobilisation. Resultant composite element has one functional P\T gene and two copies of ry+t7.2.

      (Robertson, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      P\TΔ2-3
      Δ2-3
      (Banga et al., 1991)
      Δ2-3(99B)
      (Engels et al., 1990)
      Name Synonyms
      Secondary FlyBase IDs
        References (43)