FB2024_03 , released June 25, 2024
Recombinant Construct: H{PΔ2-3}
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General Information
Symbol
H{PΔ2-3}
FlyBase ID
FBtp0001399
Feature type
transgenic_transposable_element
Component Allele(s)
P\TΔ2-3
Size
Expression Data
Associated insertion(s)
4 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele P\TΔ2-3
Transgenic product class
Component
Use(s)
Regulatory region(s)
P\T
Encoded product / tool
P\T
Description

P\TΔ2-3 lacks a tissue-specific intron that normally limits transposase activity to the germline.

(Engels et al., 1990)

The last intron, which is responsible for the limitation of transposase activity to the germline, has been deleted. P\TΔ2-3 therefore produces transposase in both the somatic and germ cells.

(Ronsseray et al., 1991)

Precise deletion of the intron sequences at the ORF2 and ORF3 junction.

(Laski et al., 1986)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pH{PΔ2-3}
(Calvi, 1993)
descendant(s)
Comments

report comment: Note: Identity of promoter as Hsp70 may be suspect; see report for pUChsΔ2-3. Heat shock not necessary for high levels of expression.

(Calvi, 1993)

construct_comment: Constructed in W. Gelbart lab.

(Indiana University, 1992-)

construct_comment: Source virtually immobile upon exposure to P-element transposase (appears to be an extremely low level of cross-mobilization, thus not completely stable).Able to transposase upon exposure to H-element transposase."HOP" for "hobo on P" or "hobo outside P".

(Calvi, 1993)
Stocks (6)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
H[w+, HOP]
(Calvi, 1993)
H{P{Δ2-3}}
(FlyBase, 1996-)
H{PΔ2-3}
(FlyBase, 1996-)
H{w+, Δ2-3}
(McVey et al., 2004)
Secondary FlyBase IDs
    References (4)