P\TΔ2-3 lacks a tissue-specific intron that normally limits transposase activity to the germline.
The last intron, which is responsible for the limitation of transposase activity to the germline, has been deleted. P\TΔ2-3 therefore produces transposase in both the somatic and germ cells.
Precise deletion of the intron sequences at the ORF2 and ORF3 junction.
P-element sequences from base pair 38 to base pair 2868 in the canonical sequence, with the intron between exons 2 and 3 removed, have been cloned into the H{hawN} vector. The P\T transposase encoded by H{w+.Δ2-3.M} cannot catalyse its own movement as the P-element ends are truncated.
Transposase activity encoded by H{w+.Δ2-3.M} is not maternally transmitted and germ-line transposase activity encoded by H{w+.Δ2-3.M} is repressed by the P cytotype.