FlyBase Allele Report: Dmel\CycE[hs.PO]

General Information

Symbol

Dmel\CycE^hs.PO

Species

D. melanogaster

Name

heat shock construct of O'Keefe

FlyBase ID

FBal0033579

Feature type

allele

Associated gene

Dmel\CycE

Associated Insertion(s)

Carried in Construct

P{hs-DmcycE}

Also Known As

HS-cyclin E

Key Links

Transgenic product class

wild_type

(Milan et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Milan et al., 1996)

Mutagen

in vitro construct

(Calvi et al., 1998, Su and O'Farrell, 1998, Milan et al., 1996, Knoblich et al., 1994)

Progenitor genotype

Carried in construct

P{hs-DmcycE}

Cytology

Description

CycE is expressed under the control of an Hsp70 promoter.

(Milan et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

CycE

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | oogenesis

(Lilly and Spradling, 1996)

abnormal mitotic cell cycle

(Ohlmeyer and Schupbach, 2003)

increased occurrence of cell division | embryonic stage | heat sensitive

(Knoblich et al., 1994)

increased occurrence of cell division | pupal stage

(Milan et al., 1996)

Phenotype Manifest In

egg chamber

(Ohlmeyer and Schupbach, 2003)

embryo

(Knoblich et al., 1994)

follicle cell

(Lilly and Spradling, 1996)

nurse cell

(Lilly and Spradling, 1996)

wing blade ventral compartment

(Milan et al., 1996)

wing cell

(Milan et al., 1996)

wing margin

(Milan et al., 1996)

Detailed Description

Statement

Reference

Overexpression of CycE^hs.PO results in an extra mitotic division in 6% of egg chambers.

(Ohlmeyer and Schupbach, 2003)

When CycA^hs.PK is over-expressed in adult females no effect on germline cysts is detectable.

(Lilly et al., 2000)

CycE overproduction leads to entry into S phase only in the central domain of the anterior ZNC in the developing wing, but in the posterior ZNC most cells entered S phase.

(Johnston and Edgar, 1998)

Heat shocked females show late replication in more than 10% of BrdU-incorporating nuclei in both polyploid nurse and follicle cells.

(Lilly and Spradling, 1996)

Expression by heat shock of CycE^hs.PO in pupae 4-8 hours after puparium formation causes the wing margin cells and a few cells of the distal wing blade to enter S phase. Expression by heat shock of CycE^hs.PO in pupae 20-24 hours after puparium formation causes wing cells, except the wing margin and middle intervein cells, to enter S phase. Expression by heat shock of CycE^hs.PO in pupae 24-28 hours after puparium formation causes only the replication of intervein cells adjacent to the veins. Adult wings derived from heat shocked pupae show no increase in the number of cells compared to wild-type.

(Milan et al., 1996)

Heat shock at 37^oC for 30 minutes at stage 11 of embryogenesis triggers progression through an extra mitosis. Anaphase figures in treated embryos appear normal and do not display chromosome bridges, indicating that DNA replication in the extra S phase was complete.

(Knoblich et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

CycE^hs.PO is a suppressor of abdominal 4 ventral denticle belt | germline clone | maternal effect phenotype of hop²

(Chen et al., 2003)

CycE^hs.PO is a suppressor of abdominal 5 ventral denticle belt | germline clone | maternal effect phenotype of hop²

(Chen et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of denticle belt A5 and gap in denticle belt A4 seen in hop² germ-line clone embryos is suppressed by CycE^hs.PO, although the two rescued denticle belts tend to fuse with each other laterally.

(Chen et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CycE^hs.PO

Name Synonyms

heat shock construct of O'Keefe

(Knoblich et al., 1994)

Secondary FlyBase IDs

References (16)

Report Sections

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