FlyBase Allele Report: Dmel\rho[PΔ5]

General Information

Symbol

Dmel\rho^PΔ5

Species

D. melanogaster

Name

FlyBase ID

FBal0033087

Feature type

allele

Associated gene

Dmel\rho

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Ruohola-Baker et al., 1993)

loss of function allele

(Freeman et al., 1992)

Mutagen

Delta2-3 transposase

(Freeman et al., 1992)

Progenitor genotype

P{lwB}X81

(Freeman et al., 1992)

Cytology

Description

Deletion removing whole of P{lwB}X81 and extending to between 88 nucleotides 3' of the presumed initiating ATG and 267 nucleotides 3' to this ATG.

(Freeman et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Ruohola-Baker et al., 1993, Freeman et al., 1992)

some die during embryonic stage | recessive

(Ruohola-Baker et al., 1993, Freeman et al., 1992)

Phenotype Manifest In

abdominal lateral pentascolopidial chordotonal organ lch5

(Elstob et al., 2001)

embryonic/larval oenocyte

(Elstob et al., 2001)

embryonic/larval tracheal dorsal trunk

(Llimargas and Casanova, 1999)

embryonic/larval tracheal system

(Llimargas and Casanova, 1999)

embryonic/larval visceral tracheal branch

(Llimargas and Casanova, 1999)

embryonic trachea & cortical actin cytoskeleton

(Cela and Llimargas, 2006)

eye photoreceptor cell | somatic clone

(Yamada et al., 2003)

pale ommatidium | somatic clone

(Birkholz et al., 2009)

tracheal branch primordium

(Cela and Llimargas, 2006)

Detailed Description

Statement

Reference

Marker expression analysis shows that rho^PΔ5 homozygous eyes (generated using 'ey-FLP' + Scer\FRT) the proportion of pale-ommatidia is reduced and hybrid ommatidia are present with a yellow-type R8 and a pale-type R7. In eyes that are a mosaic of rho^PΔ5 homozygous, heterozygous and wild-type clones, there is a significant decrease in the proportion of pale-type R8 cells in pale ommatidia in ommatidia where both R8 are R7 are not rho^PΔ5 homozygous. This proportion is not significantly different in ommatidia with a rho^PΔ5 homozygous R7 and wild-type of heterozygous R8, but is decreased significantly more in ommatidia with a rho^PΔ5 homozygous R8, irrespective of the genotype of R7.

(Birkholz et al., 2009)

The tracheal system of rho^PΔ5 embryos show branch interruptions and branches with cells only connected by cytoplasmic extensions. The tracheal tubes of stage 16 rho^PΔ5 embryos show a thinner accumulation of cortical actin compared to controls.

(Cela and Llimargas, 2006)

Ommatidia in homozygous clones sometimes have missing photoreceptor cells.

(Yamada et al., 2003)

Homozygous clones in the adult abdomen do not show any consistent alterations of normal polarity.

(Lawrence et al., 2002)

The number of chordotonal organs in the lateral cluster is reduced from 5 to 3 in stage 16 homozygous embryos and there is a complete lack of oenocytes. Oenocyte precursor whorls are missing in stage 11 embryos.

(Elstob et al., 2001)

Homozygous embryos show a variable tracheal phenotype consisting mostly of defects in migration along the anteroposterior axis. The dorsal trunk and visceral branches are missing or incomplete. Some tracheal cells remain in the ectoderm at stage 12 in contrast to wild type.

(Llimargas and Casanova, 1999)

Embryos show normal guts.

(Szuts et al., 1998)

No effect on the faf eye phenotype.

(Huang and Fischer-Vize, 1996)

Germline clone experiments show that rho has no function in oogenesis.

(Ruohola-Baker et al., 1993)

Defects in embryonic head skeleton, and characteristic fusions of denticle belts.

(Freeman et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

rho^PΔ5/rho^PΔ5 is a non-suppressor of photoreceptor cell R8 of pale ommatidium phenotype of Egfr^E1

(Birkholz et al., 2009)

Other

Statement

Reference

Gug¹⁴⁹⁶⁷, rho^PΔ5, vn¹⁰⁵⁶⁷ has wing vein | somatic clone phenotype

(Charroux et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Although extra wing vein material is induced in Gug¹⁴⁹⁶⁷ clones, wing vein differentiation is blocked in vn¹⁰⁵⁶⁷ rho^PΔ5 Gug¹⁴⁹⁶⁷ triple mutant clones.

(Charroux et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Fails to complement rho^7M43.

(Freeman et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

pΔ5

(Ruohola-Baker et al., 1993)

rho-1^PΔ5

(Wasserman et al., 2000)

rho^-

(Lawrence et al., 2002)

rho^PD5

(Huang and Fischer-Vize, 1996)

rho^PΔ5

(Jussen et al., 2016, Sato et al., 2016, Birkholz et al., 2009, Maybeck and Röper, 2009, Duong et al., 2008, Cela and Llimargas, 2006, Charroux et al., 2006)

rhom^PΔ5

(Dubois et al., 2001)

ve^rho-PΔ5

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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