FB2024_03 , released June 25, 2024
Allele: Dmel\hdFf
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General Information
Symbol
Dmel\hdFf
Species
D. melanogaster
Name
FlyBase ID
FBal0031893
Feature type
allele
Associated gene
Dmel\hd
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl nitrosourea
(Carpenter, 1999, Carpenter, 1994)
Progenitor genotype
Cytology

Polytene chromosomes normal.

(Carpenter, 1999)
Description

Amino acid replacement: W246term.

(Bandura et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:5,373,645..5,373,645 [-]
Nucleotide change:

G5373645A

Amino acid change:

W246term | hd-PA; W246term | hd-PB

Reported amino acid change:

W246term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Bandura et al., 2005, Carpenter, 1999, Carpenter, 1994)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Imaginal discs of hdFf/Df(3R)3-4 transheterozygous larvae have cell proliferation defects.

      Clones of homozygous hdFf follicle cells arrest during G2 and early M phases of the cell cycle without evidence of chromosome bridging.

      (Lesly et al., 2017)

      hdFf/Df(3R)3-4 mutants have small brains that display severely reduced levels of DNA synthesis compared to wild type. They also have no identifiable imaginal discs and small, under-replicated polytene chromosomes in the salivary glands. hdFf mutant clones generated in the wing disc are significantly smaller both in terms of size and number of cells than wild-type twin spots.

      (Bandura et al., 2005)

      FACS analysis shows that a higher proportion of hdFf cells from wing disc clones have a 2C or intermediate 2C-4C content than wild-type cells. BrdU incorporation shows that DNA replication is reduced within the hdFf clones. Additionally, cells from the hdFf wing disc mutant clones show a higher proportion of repair foci than wild-type twin spots.

      (Bandura, 2005)

      Early pupal lethal.

      (Carpenter, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Df(3R)3-4/hdFf is rescued by hdEGFP

      (Lesly et al., 2017)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      A.T.C. Carpenter.

      (Carpenter, 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      hdFf
      (Lesly et al., 2017, Becker et al., 2016)
      l(3)82Ff1
      (Carpenter, 1999, Carpenter, 1994)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)