FlyBase Allele Report: Dmel\grk[2E12]

General Information

Symbol

Dmel\grk^2E12

Species

D. melanogaster

Name

FlyBase ID

FBal0031405

Feature type

allele

Associated gene

Dmel\grk

Associated Insertion(s)

Carried in Construct

Also Known As

grk^2E, grk^E12

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neuman-Silberberg and Schupbach, 1996, Neuman-Silberberg and Schupbach, 1993)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G2235A.

(Thio et al., 2000)

Amino acid replacement: W227term.

(Thio et al., 2000, Queenan et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,431,882..8,431,882 [-]

Nucleotide change:

G8431882A

Reported nucleotide change:

G2235A

Amino acid change:

W228term | grk-PA

Reported amino acid change:

W227term

(Thio et al., 2000, Queenan et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Neuman-Silberberg and Schupbach, 1993)

Phenotype Manifest In

dorsal appendage

(Filardo and Ephrussi, 2003)

dorsal appendage (with grk^2B6)

(Niepielko and Yakoby, 2014)

egg

(Queenan et al., 1997)

egg chorion (with grk^2B6)

(Niepielko and Yakoby, 2014)

embryo

(Queenan et al., 1999)

micropyle

(Queenan et al., 1997)

pericentriolar material & oocyte | posterior

(Shulman et al., 2000)

Detailed Description

Statement

Reference

Approximately 80% of grk^2E12/+ females lay eggs with fusion of dorsal appendages.

(Filardo and Ephrussi, 2003)

The posterior follicle cell phenotype of Mer^ts1 is suppressed entirely by grk^2E12.

(MacDougall et al., 2001)

The posterior MTOC fails to disassemble in mutant oocytes, resulting in a focus of microtubules at the posterior pole.

(Shulman et al., 2000)

Embryos laid by homozygous grk^2B6 mothers are strongly ventralised.

(Queenan et al., 1999)

Eggs produced by homozygous females have a double micropyle.

(Queenan et al., 1997)

grk^2B6/grk^2E12 egg chambers show a duplication of all three anterior follicle cell types at the posterior pole.

(Gonzalez-Reyes et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

grk^2B6/grk^2E12 has egg chorion phenotype, suppressible | partially by Dwil\GK18382^+ta

(Niepielko and Yakoby, 2014)

grk^2B6/grk^2E12 has dorsal appendage phenotype, suppressible | partially by Dwil\GK18382^+ta

(Niepielko and Yakoby, 2014)

grk^2E12 has dorsal appendage phenotype, suppressible by bru1^QB/aret[+]

(Filardo and Ephrussi, 2003)

Suppressor of

Statement

Reference

grk^2E12 is a suppressor of egg chamber | posterior & follicle cell phenotype of Mer^ts1

(MacDougall et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for aret^QB reduces the penetrance of dorsal appendage loss or fusion phenotypes in eggs laid by grk^2E12/+ females, from 80% to 40%.

(Filardo and Ephrussi, 2003)

Xenogenetic Interactions

Statement

Reference

The presence of one or two copies of Dwil\GK18382^+ta in grk^2B6/grk^2E12 mutant D. melanogaster flies results in four distinct eggshell phenotypes. Most eggshells, 69% and 78% from one and two copies of Dwil\GK18382^+ta, respectively, are wild-type or wild-type with an enlarger area posterior to the base of the dorsal appendages, similar to wild-type flies expressing Dwil\GK18382^+ta. A dorsal ridge-like morphology is found in 10% and 1% of the eggshells from one and two copies of Dwil\GK18382^+ta, respectively.

In 13% and 18% of the eggshells from in grk^2B6/grk^2E12 mutant D. melanogaster with one and two copies of Dwil\GK18382^+ta, respectively, there is a single or fused dorsal appendage.

In 8% and 3% of the eggshells from in grk^2B6/grk^2E12 mutant D. melanogaster with one and two copies of Dwil\GK18382^+ta, respectively, the eggshells are collapsed and the eggs unmountable.

(Niepielko and Yakoby, 2014)

Complementation and Rescue Data

Fails to complement

grk^ΔFRT

(Lan et al., 2010)

Rescued by

grk^2B6/grk^2E12 is rescued by grk^ΔC

(Herpers and Rabouille, 2004)

Not rescued by

grk^2B6/grk^2E12 is not rescued by grk^ΔTC

(Herpers and Rabouille, 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

grk E12

(Queenan et al., 1999)

grk^2E12

(Mallart et al., 2024, Doerflinger et al., 2022, Trovisco et al., 2016, Morais-de-Sá et al., 2013, Dubin-Bar et al., 2011, Besse et al., 2009, Krauss et al., 2009, Krauss et al., 2009, Doerflinger et al., 2006)

grk^2E12b

(Niepielko and Yakoby, 2014)

grk^2E

(Lan et al., 2010, Dollar et al., 2002)

grk^E12

(Queenan et al., 1999, Queenan et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (26)

Report Sections

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