FB2024_03 , released June 25, 2024
Allele: Dmel\βTub85D432
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General Information
Symbol
Dmel\βTub85D432
Species
D. melanogaster
Name
FlyBase ID
FBal0028628
Feature type
allele
Associated gene
Dmel\βTub85D
Associated Insertion(s)
Carried in Construct
P{B2t.432}
Key Links
Genomic Maps

Transgenic product class
stop_gained
(Fackenthal et al., 1993)
Mutagen
Nature of the Allele
Transgenic product class
stop_gained
(Fackenthal et al., 1993)
Mutagen
in vitro construct
(Fackenthal et al., 1995, Fackenthal et al., 1993)
Progenitor genotype
βTub85D+t4.55
(Fackenthal et al., 1995)
Carried in construct
P{B2t.432}
Cytology
Description

Stop codons introduced at codon positions 432 and 433, results in deletion of the last 15 amino acids of the C terminus.

(Fackenthal et al., 1995)

Stop codons introduced in the coding region in codon positions 432 and 433, by two T for G substitutions.

(Fackenthal et al., 1993)
Allele components
Component
Use(s)
Regulatory region(s)
βTub85D
Encoded product / tool
βTub85D
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:9,408,700..9,408,700 [-]
Nucleotide change:

G9408700T

Amino acid change:

E432term | betaTub85D-PA

Reported amino acid change:

E432term

(Hoyle et al., 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Males carrying one or two copies of βTub85D432 in a wild-type background are fertile. Males carrying four copies of βTub85D432 in a wild-type background are fertile, but have reduced fecundity and a reduced amount of motile sperm compared to wild type. Males carrying one or two copies of βTub85D432 in a βTub85Dn/βTub85Dn background are sterile and axonemes are not produced. Males carrying one copy of βTub85D432 in a βTub85Dn/+ background are sterile.

      (Hoyle et al., 2001)

      In the absence of wild type βTub85D, βTub85D432 can support the assembly and function of many classes of microtubules but causes intrinsic defects of differing severity in meiosis, sperm head shaping and axoneme morphogenesis.

      (Fackenthal et al., 1995)

      Spermatids have normal early post-meiotic morphology. However meiotic divisions are often flawed, due to a failure in cytokinesis. Differing size of nuclei indicate non-disjunction. Spermatid heads remain unshaped. Spermatid elongation proceeds, at least to some degree, though ultrastructural examination reveals that the axoneme is abnormal.

      (Fackenthal et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Males carrying one copy of αTub84B+t4.5 and one copy of βTub85D432 in an otherwise wild-type background are sterile. Males carrying one copy of αTub85EβTub85D.PH and one copy of βTub85D432 in an otherwise wild-type background are sterile. Df(3R)Scx4/+ ; βTub85Dn/+ males carrying one copy of βTub85D432 are fertile. Males carrying one copy of βTub56DβTub85D.PR and one copy of βTub85D432 in a βTub85Dn/+ background are fertile, but have reduced fecundity and a reduced amount of motile sperm compared to wild type. Males carrying one copy of βTub60D::βTub85DβTub85D.3'UTR and one copy of βTub85D432 in a βTub85Dn/+ background are sterile and have abnormal axonemes.

      (Hoyle et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      βTub85D432 fails to rescue βTub85Dn

      (Nielsen et al., 2001)
      Comments

      Males carrying one copy of P{B2t.432} in a hemizygous background (βTub85Dn/βTub85D+) are sterile.

      (Fackenthal et al., 1995)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Protein product (B2ΔC) can be assembled into ultrastructurally normal looking microtubules in vivo. Phenotypes of males with 1 to 4 copies of this allele are identical.

      (Fackenthal et al., 1993)

      Encodes the protein product B2ΔC.

      (Fackenthal et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      B2tΔC
      (Hoyle et al., 1995)
      B2ΔC
      (Wilson and Borisy, 1997)
      β2ΔC
      (Nielsen et al., 2001)
      βTub85D432
      Name Synonyms
      Secondary FlyBase IDs
        References (6)