FlyBase Allele Report: Dmel\ph-p[lac]

General Information

Symbol

Dmel\ph-p^lac

Species

D. melanogaster

Name

FlyBase ID

FBal0024620

Feature type

allele

Associated gene

Dmel\ph-p

Associated Insertion(s)

P{lacW}ph-p^lac

Carried in Construct

Also Known As

ph^lac

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Fauvarque et al., 1995)

Mutagen

P-element activity

(Fauvarque et al., 1995, Fauvarque and Dura, 1994)

Progenitor genotype

Associated Insertion(s)

P{lacW}ph-p^lac

Cytology

Description

P{lacW} insertion is close to the first intron splice site.

(Fauvarque et al., 1995)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis

(Narbonne et al., 2004)

abnormal mitotic cell cycle

(Narbonne et al., 2004)

viable

(Fauvarque et al., 1995)

Phenotype Manifest In

egg chamber

(Narbonne et al., 2004)

germarium

(Narbonne et al., 2004)

germarium region 3

(Narbonne et al., 2004)

germline cyst

(Narbonne et al., 2004)

oocyte

(Narbonne et al., 2004)

polar follicle cell | ectopic

(Narbonne et al., 2004)

stalk follicle cell

(Narbonne et al., 2004)

stalk follicle cell | ectopic

(Narbonne et al., 2004)

Detailed Description

Statement

Reference

ph-p^lac homozygous females have egg chambers with abnormal numbers of germ cells. Egg chambers in these mutants with more than 16 germ cells (23% ovarioles contain at least one of these) sometimes contain groups of nurse cells with differing degrees of polyploidy, but all contain multiple oocytes. When an egg chamber contains fewer than 15 nurse cells (5.6%), one of the adjacent chambers contains the complementary number of nurse cells or degenerating nurse cells. Whether follicles contain more or fewer than the normal number of germline cells, there are always two groups of polar cells, one at each extremity, anterior and posterior, but these groups often contain extra polar cells. Apposed eggs chambers not separated by an interfollicular stalk are also present in these mutants. Where stalks are present they are abnormal - containing 10-50 cells in 2-3 lines rather than five to seven cells organized in a single line as in wild-type. This excess of polar and stalk cells is not due to any proliferation of these cells after stage 1, and so must be due to processes occurring in the germarium. Germaria in ph-p^lac homozygous females display a variety of phenotypes: delayed budding of the emerging egg chambers; disorganized arrangement of encapsulated cysts; splitting of germline cysts and envelopment of fewer than 16 germline cells; an absence of prefollicular cell migration between adjacent germline cysts associated with an accumulation of several mature cysts in region 3 of the germarium (in these cases, prefollicular cell invagination can be seen anteriorly, probably prefiguring to budding of a multicyst egg chamber). In the most extreme cases there is no clear boundary between germarium and vitellarium. The number of mitotic somatic cells in regions 2b and 3 of the germarium is significantly lower for ph-p^lac homozygous females compared to ph-p^lac/+ control sisters.

(Narbonne et al., 2004)

Transheterozygotes with a null ph-p allele or a deficiency of ph-p cause lethality.

(Fauvarque et al., 1995)

Mild homeotic transformation.

(Serrano et al., 1995)

Homozygous viable.

(Fauvarque and Dura, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

ph-p^lac/ph-p[+] is a non-enhancer of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

ph-p^lac/ph-p[+] is a non-enhancer of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

NOT Suppressor of

Statement

Reference

ph-p^lac/ph-p[+] is a non-suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

ph-p^lac/ph-p[+] is a non-suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

Other

Statement

Reference

en¹, ph-p^lac has visible | recessive phenotype

(Maschat et al., 1998)

en¹/en⁵⁴, ph-p^lac has visible | recessive phenotype

(Maschat et al., 1998)

en^Xho25, ph-p^lac has visible | recessive phenotype

(Maschat et al., 1998)

en⁵⁴, ph-p^lac has visible | dominant phenotype

(Maschat et al., 1998)

en^Xho25, ph-p^lac has visible | dominant phenotype

(Maschat et al., 1998)

en⁵⁴, ph-p^lac has visible | recessive phenotype

(Maschat et al., 1998)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

ph-p^lac/ph-p[+] is a non-enhancer of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

ph-p^lac/ph-p[+] is a non-enhancer of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

NOT Suppressor of

Statement

Reference

ph-p^lac/ph-p[+] is a non-suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

ph-p^lac/ph-p[+] is a non-suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

Other

Statement

Reference

en¹, ph-p^lac has wing vein L4 phenotype

(Maschat et al., 1998)

en¹/en⁵⁴, ph-p^lac has wing vein L4 phenotype

(Maschat et al., 1998)

en⁵⁴, ph-p^lac has wing vein L4 phenotype

(Maschat et al., 1998)

en^Xho25, ph-p^lac has wing vein L4 phenotype

(Maschat et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is not affected by combination with a single copy of ph-p^lac.

(Morimoto et al., 2016)

0% of ph-p^lac/+; en⁵⁴/+ double heterozygotes and 100% of ph-p^lac/Y; en⁵⁴/+ double heterozygotes have a gap in the fourth wing vein at 25^oC. 100% of ph-p^lac/Y ; en¹/en¹ and ph-p^lac/Y ; en¹/en⁵⁴ flies have a gap in the fourth wing vein at 25^oC. 20% of ph-p^lac/Y ; en^Xho25/+ flies have a gap in the fourth wing vein at 25^oC.

(Maschat et al., 1998)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is not affected by combination with a single copy of ph-p^lac.

(Morimoto et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5443

P{lacW}ph-p^lac w¹¹¹⁸

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ph-p^lac

ph^lac

(Narbonne et al., 2004, Maschat et al., 1998, Fauvarque et al., 1995, Fauvarque and Dura, 1994)

Name Synonyms

Secondary FlyBase IDs

References (6)

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