FlyBase Allele Report: Dmel\Sxl[f5]

General Information

Symbol

Dmel\Sxl^f5

Species

D. melanogaster

Name

FlyBase ID

FBal0016683

Feature type

allele

Associated gene

Dmel\Sxl

Associated Insertion(s)

Carried in Construct

Also Known As

Sxl^fs2

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Mutation falls in the carboxy-terminal part of the protein, well downstream of the second Sxl RNA recognition motif, in a stretch of 7 proline residues interrupted by a single glutamine.

(Bopp et al., 1993)

Amino acid replacement: P315L.

(Bopp et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:7,084,646..7,084,646 [-]

Nucleotide change:

C7084646T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

P315L

Comment:

A change from CCA (P) to CTA (L) in the P directly preceding the Q in the span PPPPPQPP.

(Bopp et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Bopp et al., 1999, Sass et al., 1995, Bae et al., 1994, Geyer et al., 1993, Mohler and Carroll, 1984)

female sterile (with T(1;1)SxlΔPm)

(Evans and Cline, 2013)

female sterile | recessive

(Starr and Cline, 2002, Bopp et al., 1993)

viable

(Starr and Cline, 2002)

Phenotype Manifest In

ovary

(Starr and Cline, 2002, Bae et al., 1994, Steinmann-Zwicky, 1994, Bopp et al., 1993, Mohler and Carroll, 1984)

Detailed Description

Statement

Reference

Homozygous females are viable but sterile. 100% of ovaries of homozygous females that are not infected with Wolbachia have no eggs. This oogenesis defect is partly rescued by Wolbachia infection; 43% of the ovaries of infected homozygous females have no eggs, 25% of ovaries have 1-2 eggs, 25% of ovaries have 3-9 eggs and 7% of ovaries have more than 10 eggs. 70% of ovaries of Sxl^f5/Sxl^f1 females that are infected with Wolbachia have no eggs, 5% of ovaries have 1-2 eggs, 15% of ovaries have 3-9 eggs and 10% of ovaries have more than 10 eggs.

(Starr and Cline, 2002)

Sxl^f4/Sxl^f5; Su(Sxl^fs)46⁴⁶/+ females show severely reduced recombination and impaired segregation of homologous chromosomes. The reduction in recombination is caused by the lack of wild-type Sxl activity rather than the presence of Su(Sxl^fs)46⁴⁶.

(Bopp et al., 1999)

XX tra¹/tra⁴ pseudomales that are also homozygous for Sxl^f5 show a decrease in the frequency of oogenic gonads and an increase in the frequency of non-oogenic gonads compared to XX tra¹/tra⁴ pseudomales. Atrophic gonads are seen at the same frequency as XX tra¹/tra⁴ pseudomales. Oogenic gonads are not seen in XX tra¹/tra⁴ pseudomales carrying otu^hs.PN that are also homozygous for Sxl^f5. In each case the frequency of non-oogenic gonads is increased compared to XX tra¹/tra⁴ pseudomales also homozygous for Sxl^f5.

(Hinson and Nagoshi, 1999)

Tumorous ovaries.

(Bae et al., 1994)

Sxl^f1/Sxl^f5 female larvae have gonads that are female-like in size and morphology, although Sxl^f1/Sxl^f5 adult females have ovaries filled with male-like multicellular cysts.

(Steinmann-Zwicky, 1994)

Persistent cytoplasmic accumulation of Sxl protein in the germ line.

(Bopp et al., 1993)

No eggs are laid, ovaries are rudimentary.

(Mohler and Carroll, 1984)

fully viable female-sterile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sxl^f1/Sxl^f5 has female sterile phenotype, suppressible | partially by Oda[+]/Oda^k11803

(Vied et al., 2003)

Sxl^f5 has female sterile phenotype, suppressible by Su(Sxl^fs)46⁴⁶

(Bopp et al., 1999)

Sxl^f5 has female sterile phenotype, suppressible by Su(Sxl^fs)X2^X2

(Bopp et al., 1999)

Other

Statement

Reference

Su(Sxl^fs)46⁴⁶, Sxl^f5 has female fertile phenotype

(Bopp et al., 1999)

Su(Sxl^fs)X2^X2, Sxl^f5 has female fertile phenotype

(Bopp et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sxl^f1/Sxl^f5 has ovary phenotype, suppressible | partially by Oda[+]/Oda^k11803

(Vied et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Oda^k11803/+ partially suppresses the ovary phenotype and female sterility of Sxl^f5/Sxl^f1.

(Vied et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

4598

y¹ cv¹ Sxl^f5 v¹ f¹/FM3

Notes on Origin

Discoverer

Comments

Small amount of male-specific Sxl splicing in ovaries. Sxl product distribution is abnormal: transition to nuclear staining is not made.

(Bopp et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

M106

(Perrimon et al., 1986)

Sxl^f5

(Gawande et al., 2006)

Sxl^fs2

(Chang et al., 1999, Bloomington Drosophila Stock Center, 1998.3.2, Geyer et al., 1993)

(Evans and Cline, 2013)

fs(1)M106^13-558

(Bloomington Drosophila Stock Center, 1998.3.2, Mohler and Carroll, 1984)

fs(1)M106²

(Bloomington Drosophila Stock Center, 1998.3.2)

fs2

Name Synonyms

Secondary FlyBase IDs

FBal0030318

References (18)

Report Sections

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