FlyBase Allele Report: Dmel\stnA[7]

General Information

Symbol

Dmel\stnA⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0016207

Feature type

allele

Associated gene

Dmel\stnA

Associated Insertion(s)

Carried in Construct

Also Known As

stn^ts2

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Petrovich et al., 1993)

Progenitor genotype

Cytology

Description

Nucleotide substitution: A104T. Amino acid replacement: K35M.

(Phillips et al., 2000)

An amino acid replacement - single substitution in stnA.

(Stimson et al., 1998)

Lesion may map to the stnA rather than the stnB transcription unit.

(Kelly, 1997.1.23)

Contains the InsOR20B 2.4kb insertion polymorphism present in Oregon-R but not Canton-S.

(Andrews et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:22,995,656..22,995,656 [-]

Nucleotide change:

A22995656T

Reported nucleotide change:

A104T

Amino acid change:

K35M | stnA-PC; K35M | stnA-PD; K35M | stnA-PE; K35M | stnA-PF

Reported amino acid change:

K35M

(Phillips et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

stnA⁷ larvae show approximately threefold enhancement in the frequency of spontaneous miniature events at the neuromuscular junction compare to wild-type. Postsynaptic responses in the muscle evoked by motor nerve stimulation are not substantially altered compared to wild-type. The fractional area occupied by vesicles is not significantly different from wild-type in stnA¹⁵ boutons at the neuromuscular junction, although there is a trend towards wider synaptic vesicle distribution than in wild-type. The synaptic vesicles are indistinguishable from wild-type and synaptic vesicle density is normal. The number of boutons per arbor is slightly, but significantly, increased compared to wild-type.

(Stimson et al., 1998)

At 22^oC flies walk slowly, occasionally fall over, flutter wings, can climb and fly. At 29^oC flies fall to the bottom of the vial, are paralyzed, though incompletely, as their legs kick as they lie on their backs. Mutant phenotype is reversible by shifting back to low temperature. Heterozygotes with Df(1)30A emerge late, become mired in the medium, and show low relative viability. ERG phenotype shows increased amplitude of off-transient. Flies heterozygous for a ts and a lethal allele of stnA show ERGs with off transient amplitudes between those for stnA⁷ and stnA¹⁵. Flies heterozygous for stnA¹⁵ and a ts allele show ERG off transients significantly lower than for the control class, and lower than for the heterozygotes between ts and lethal alleles of stnA.

(Petrovich et al., 1993)

Unusual jumping reaction in response to a light off stimulus and unable to coordinate movements at the restrictive temperature.

(Kelly et al., 1987)

Homozygous flies can walk at the restrictive temperature, but do so with difficulty.

(Miklos et al., 1987)

Severe behavioral debilitation is quickly induced by shift of adult stnA⁷ from 25^oC to 29^oC; abnormalities at high temperature include uncoordinated leg and wing movements, with complete paralysis never occurring (e.g., legs still move); stnA⁷ is more nearly normal at low temperatures and can even walk with difficulty at 29^oC; at permissive temperatures.

(Kelly, 1983)

temperature-sensitive behavioral mutant

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dnc¹, stnA⁷ has lethal | recessive phenotype, suppressible by Su(stn)¹

(Petrovich et al., 1993)

Other

Statement

Reference

Syt1^D27/Syt1^AD4, Syt1^elav.PD, stnA⁷ has lethal phenotype

(Phillips et al., 2000)

Syt1^D27/Syt1^AD4, Syt1^elav.PD, stnA⁷ has uncoordinated phenotype

(Phillips et al., 2000)

shi^unspecified, stnA⁷ has abnormal behavior phenotype

(Andrews et al., 1995)

dnc^unspecified, stnA⁷ has abnormal behavior phenotype

(Andrews et al., 1995)

dnc¹, stnA⁷ has lethal | recessive phenotype

(Petrovich et al., 1993)

dnc^M14, stnA⁷ has lethal | recessive phenotype

(Petrovich et al., 1993)

shi¹, stnA⁷ has lethal | recessive phenotype

(Petrovich et al., 1993)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The viability of syt^D27/syt^AD4 flies (rescued to adulthood by syt^elav.PD) is significantly reduced in combination with stnA⁷/Y. The few surviving males have an extreme behavioural phenotype and are very sedentary and severely uncoordinated (they are more uncoordinated than stnA⁺/Y ; syt^D27/syt^AD4 flies rescued to adulthood by syt^elav.PD). They die within 48 hours of eclosion.

(Phillips et al., 2000)

Allele specific synergistic interaction with mutations at the shi and dnc loci.

(Andrews et al., 1995)

The lethality of shi¹, stnA⁷ double mutants is complete. The Sh⁷, stnA⁷, para^ts1, stnA⁷ and rut^-,stnA⁷ double mutants are no more inviable than stnA⁷ alone. Su(stn)¹ suppresses the lethal interaction of dnc¹, stnA⁷.

(Petrovich et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

stnA¹⁵

(Stimson et al., 1998)

stnA¹⁵

(Petrovich et al., 1993)

Fails to complement

stnA¹²

(Stimson et al., 1998)

Comments

Fails to fully complement the reduced excitatory junctional potential amplitude of stnA¹⁵.

(Stimson et al., 1998)

stnA¹⁵ entirely complements the uncoordinated phenotype of stnA⁷, though stnA⁷ does not complement the stnA¹⁵ stress-sensitive behavior.

(Petrovich et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Putative cluster-mate of stnA⁶; both stnA⁶ and stnA⁷ were isolated from the same strain and have the same nucleotide substitution.

(Phillips et al., 2000)

Comments

Augments the synaptic output.

(Andrews et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

stn⁷

stn^ts2

(Phillips et al., 2000, Stimson et al., 1998, Kelly, 1997.1.23, Andrews et al., 1996, Andrews et al., 1995, Petrovich et al., 1993, Kelly et al., 1987, Miklos et al., 1987)

stnA⁷

stnTS2

(Fergestad and Broadie, 1997)

Name Synonyms

Secondary FlyBase IDs

References (11)

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