FlyBase Allele Report: Dmel\spn-D[2]

General Information

Symbol

Dmel\spn-D²

Species

D. melanogaster

Name

FlyBase ID

FBal0016040

Feature type

allele

Associated gene

Dmel\spn-D

Associated Insertion(s)

Carried in Construct

Also Known As

spn-D³⁴⁹, spnD³⁴⁹

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Gonzalez-Reyes et al., 1997)

loss of function allele

(Ghabrial et al., 1998)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Bhagat et al., 2004)

abnormal meiotic cell cycle (with spn-D¹)

(Bhagat et al., 2004)

semi-sterile

(Bhagat et al., 2004)

semi-sterile (with spn-D¹)

(Bhagat et al., 2004)

viable

(Ghabrial et al., 1998)

Phenotype Manifest In

dorsal appendage

(Klattenhoff et al., 2007, Gonzalez-Reyes et al., 1997)

egg | dorsal

(Gonzalez-Reyes et al., 1997)

egg chorion | maternal effect

(Abdu et al., 2003)

egg chorion | maternal effect (with spn-D¹)

(Abdu et al., 2003)

germline cyst

(Huynh and St. Johnston, 2000)

karyosome

(Gonzalez-Reyes et al., 1997)

karyosome (with spn-D¹)

(Abdu et al., 2003)

microtubule organizing center & oocyte

(Klattenhoff et al., 2007)

nurse cell

(Gonzalez-Reyes et al., 1997)

oocyte

(Gonzalez-Reyes et al., 1997)

presumptive oocyte

(Joyce and McKim, 2009)

synaptonemal complex

(Huynh and St. Johnston, 2000)

Detailed Description

Statement

Reference

Homozygous females show a high frequency (more than 50%) of region 3 cysts with two pro-oocytes (as assayed by c(3)G staining) compared to a frequency of only 9.5% in wild type.

(Joyce and McKim, 2009)

In spn-D² oocytes, both the anterior and later posterior microtubule-organizing centers are much less prominent than in wild-type oocytes.

Over half of spn-D² eggs show a fused dorsal appendage phenotype and 11% of eggs have no dorsal appendages at all.

(Klattenhoff et al., 2007)

spn-D²/spn-D¹ larvae are not sensitive to MMS.

(McCaffrey et al., 2006)

spn-D²/spn-D² females exhibit low fertility, and meioses show reduced frequency of crossing over along most of chromosome 2, but not in the centromeric regions, as compared to wild type.

spn-D²/spn-D¹ females exhibit low fertility, and meioses show reduced frequency of crossing over along most of chromosome 2, as compared to wild type.

(Bhagat et al., 2004)

43% of eggs derived from homozygous females have wild-type eggshells, 57% have mutant eggshells, with 21% having dorsalised eggs with dorsal appendage material around the lateral and ventral side of the egg. 70% of eggs derived from spn-D¹/spn-D² females have mutant eggshells and defects in karyosome morphology are seen in the mutant egg chambers.

(Abdu et al., 2003)

In contrast to wild-type ovaries, where the synaptonemal complex (SC) is always restricted to the oocyte by region 2b, spn-D² mutant females show a significant delay in the process, with cysts with more than 1 cell in synapsis in region 3 of the germarium and one cell in synapsis in stage 2 of the vitellarium.

(Huynh and St. Johnston, 2000)

Hemizygotes are viable. spn-D¹/spn-D² larvae show normal sensitivity to methyl methanesulfonate. Recombination frequency is 10-25% of normal levels in homozygous females, and X chromosome non-disjunction is increased 100-fold compared to wild-type.

(Ghabrial et al., 1998)

Hemizygous eggs exhibit either a strong or weak ventralised phenotype: eggs are longer than wild type and are completely symmetric along the DV axis or the eggs display fused dorsal appendages. Egg chambers of females exhibit a partially penetrant disruption in the positioning of the oocyte which can be located anywhere in the egg chamber. Germline clones in egg chambers are composed of wild type follicle cells, mutant nurse cells, misplaced oocyte, oocytes that give rise to ventralised eggs and oocytes that lack a karyosome.

(Gonzalez-Reyes et al., 1997)

strong

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

spn-D² has phenotype, enhanceable by spn-A³

(Huynh and St. Johnston, 2000)

spn-D² has synaptonemal complex phenotype, enhanceable by spn-A³

(Huynh and St. Johnston, 2000)

spn-D² has oocyte phenotype, enhanceable by spn-A³

(Gonzalez-Reyes et al., 1997)

spn-D² has oocyte phenotype, enhanceable by spn-B¹

(Gonzalez-Reyes et al., 1997)

spn-D² has oocyte phenotype, enhanceable by spn-E¹

(Gonzalez-Reyes et al., 1997)

Suppressed by

Statement

Reference

spn-D² has microtubule organizing center & oocyte phenotype, suppressible by lok^p6/lok^p6

(Klattenhoff et al., 2007)

spn-D² has dorsal appendage phenotype, suppressible by lok^p6/lok^p6

(Klattenhoff et al., 2007)

spn-D²/spn-D¹ has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-41^unspecified

(Abdu et al., 2003)

spn-D²/spn-D¹ has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-W68^unspecified/mei-W68^unspecified

(Abdu et al., 2003)

spn-D²/spn-D¹ has karyosome phenotype, suppressible by mei-41^D3

(Abdu et al., 2003)

spn-D²/spn-D¹ has karyosome phenotype, suppressible by mei-W68^unspecified/mei-W68^unspecified

(Abdu et al., 2003)

spn-D²/spn-D¹ has karyosome phenotype, suppressible | partially by mei-41^D1

(Abdu et al., 2003)

Enhancer of

Statement

Reference

spn-D² is an enhancer of phenotype of spn-A³

(Huynh and St. Johnston, 2000)

spn-D² is an enhancer of synaptonemal complex phenotype of spn-A³

(Huynh and St. Johnston, 2000)

spn-D² is an enhancer of oocyte phenotype of spn-B¹

(Gonzalez-Reyes et al., 1997)

spn-D² is an enhancer of oocyte phenotype of spn-E¹

(Gonzalez-Reyes et al., 1997)

Other

Statement

Reference

spn-A³, spn-D² has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The microtubule-organizing center phenotype of spn-D² oocytes is suppressed by the lok^p6 mutation.

The fused dorsal appendage phenotype of spn-D² eggs is suppressed by lok^p6.

(Klattenhoff et al., 2007)

The eggshell defects seen in eggs derived from spn-D¹/spn-D² females are completely suppressed if the females are also homozygous for mei-W68^unspecified. The karyosome defects seen in spn-D¹/spn-D² egg chambers are also suppressed by mei-W68^unspecified. The eggshell defects seen in eggs derived from spn-D¹/spn-D² females are suppressed if the females also carry mei-41^unspecified. The karyosome defects seen in spn-D¹/spn-D² egg chambers are only slightly suppressed by mei-41^D1 but are strongly suppressed by mei-41^D3.

(Abdu et al., 2003)

The phenotype of a delay in the restriction of the synaptonemal complex seen in the female germline of spn-D² or spn-A³ single mutants is enhanced in spn-D² spn-A¹ double mutants.

(Huynh and St. Johnston, 2000)

Has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males.

(McKee et al., 2000)

Double mutants with spn-A³, spn-B¹ or spn-E¹ increases the oocyte mispositioning frequency. Almost all oocytes lack a karyosome and the oocyte chromosomes are arranged instead in thread-like figures with irregular shape. Double mutant mothers with fs(1)K10¹ lay dorsalised eggs, the dorsal appendages form a collar around the entire circumference of the egg. This phenotype is indistinguishable from that of fs(1)K10¹ single mutants.

(Gonzalez-Reyes et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

spn-D^CX

(Ghabrial et al., 1998)

Rescued by

spn-D² is rescued by Scer\GAL4^{VP16.nanos.UTR}/spn-D^UASp.GFP

(Abdu et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3326

Dp(1;Y)Bar^S, Bar⁺; ru¹ Diap1^th-1 st¹ kni^ri-1 rn^roe-1 p^p e^s spn-D² ca¹/TM3, Sb¹

Kyoto

106979

Dp(1;Y)Bar^S; ru¹ Diap1^th-1 st¹ kni^ri-1 rn^roe-1 p^p e^s spn-D² ca¹/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

spn-D²

(Klattenhoff et al., 2007)

spn-D³⁴⁹

(Joyce and McKim, 2009, McCaffrey et al., 2006, Abdu et al., 2003, Findley et al., 2003, Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987)

spnD²

(McKee et al., 2000)

spnD³⁴⁹

(Bhagat et al., 2004, Huynh and St. Johnston, 2000, Ghabrial et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

Open Close