FlyBase Allele Report: Dmel\Polr2A[Ubl]

General Information

Symbol

Dmel\Polr2A^Ubl

Species

D. melanogaster

Name

Ultrabithorax-like

FlyBase ID

FBal0014778

Feature type

allele

Associated gene

Dmel\Polr2A

Associated Insertion(s)

Carried in Construct

Also Known As

Ubl

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Botas et al., 1988)

Mutagen

ethyl methanesulfonate

(Chen et al., 1993)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G4471A.

(Krasnoselskaya et al., 1998)

Amino acid replacement: D886N. Nucleotide substitution: G4471A.

(Chen et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,566,648..11,566,648 [-]

Nucleotide change:

G11566648A

Reported nucleotide change:

G4471A

Amino acid change:

D886N | RpII215-PA

Reported amino acid change:

D886N

(Chen et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Krasnoselskaya et al., 1998, Kim et al., 1994, Chen et al., 1993, Mortin et al., 1985)

visible | dominant

(Mortin et al., 1988, Mortin et al., 1985)

Phenotype Manifest In

capitellum

(Chen et al., 1993, Mortin et al., 1988)

haltere

(Mortin et al., 1985)

Detailed Description

Statement

Reference

Exhibits the Ubx effect: heterozygotes partially transform the capitellum of the haltere into the wing blade.

(Chen et al., 1993)

The capitellum of the haltere shows a partial transformation to wing blade in heterozygotes. Enhances the bristle duplication phenotype of Dl⁷ heterozygotes. 80% of RpII215/TM6 males show complete or near complete pigmentation of their fourth abdominal segments.

(Mortin et al., 1988)

Heterozygotes have a partial transformation of haltere to wing (a Ubx-like phenotype). Flies carrying both RpII215^Ubl and Ubx¹³⁰ show a greater transformation of haltere to wing than flies carrying RpII215^Ubl alone. Patches of hemizygous tissue (even in the halteres) appear morphologically normal in gynandromorphs. Mutant germ cells often differentiate into morphologically abnormal oocytes.

(Mortin et al., 1985)

Ultrabithorax-like phenotype.

(Mortin and Kaufman, 1982)

Homozygous and hemizygous lethal. Heterozygotes mimic Ubx in adding several hairs to and enlarging the

capitellum of the haltere; females homozygous for

RpII215^Ubl, surviving by virtue of a duplication for

RpII215⁺, more severely affected with capitellum

approximately three times the size of that observed in

RpII215^Ubl/+ and with two or more rows of bristles;

about 10% normal viability; poorly fertile in crosses

to wild type; sterile when crossed to RpII215^Ubl

males with a duplication of RpII215⁺. Similarly

males with one mutant and one normal allele are more

extreme than females with one mutant and two normal

alleles. Acts as a dosage sensitive enhancer of Ubx,

transforming halteres into wing-like structures;

enhancement by RpII215^Ubl/RpII215^Ubl/+ >

RpII215^Ubl/+ > RpII215^Ubl/+/+. RpII215^Ubl/+

interacts with heterozygotes for Ubx^bx-3 and Ubx^abx-1 but

not Ubx^bx-1 to produce enlarged capitellum and with

Ubx^bxd-100/+ to transform halteres into wing-like

structures; extra doses of Ubx⁺ counteract the

enhancing effects of RpII215^Ubl. A second

interaction with Ubx/+ is the production of

miscadestral-like pigmentation. Ubx enhancement by

RpII215^Ubl reduced in transheterozygotes with other

interacting alleles (Mortin, Kim and Huang, 1988). Furthermore in

RpII215^Ubl/+//RpII215^Ubl/0 gynandromorphs, the X0

tissue is without any RpII215^Ubl phenotype,

displaying neither enlarged halteres nor enhancement

of Ubx expression, whereas the XX tissue exhibits both

enlarged halteres and Ubx enhancement (Mortin,

Perrimon and Bonner, 1985). RpII215^Ubl/+ also display increased

frequencies of duplicated bristles in Dl/+ and in some

crosses causes Sb/+ flies to exhibit shortened and

broadened wings whose longitudinal veins fail to reach

the margin (Mortin, Kim and Huang, 1988). RpII215^Ubl in

heterozygous combination with deficiencies for either

ct or sno is lethal and with lethal alleles of ct

produces a strong cut phenotype; produces a mutant

phenotype of allele specific severity in heterozygous

combination with deficiencies for or lethal alleles of

br, N, dm, slc, bi, oc, m, sd and sw; interacts with

heterozygotes for mutant alleles of oc, sno and sw,

but not the others; no interaction with dor, sn, ras,

or g deficiencies. Influence of the maternal genotype

apparent since patroclinous RpII215^Ubl/Y and

RpII215^Ubl/0 males from non-mutant mothers survive

at 20% the expected rate; they are phenotypically

normal but are sterile (Voelker et al.).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S1

(Kim et al., 1994)

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S2

(Kim et al., 1994)

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S5

(Kim et al., 1994)

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S6

(Kim et al., 1994)

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S8

(Kim et al., 1994)

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S7

(Kim et al., 1994)

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S11

(Kim et al., 1994)

Polr2A^Ubl has lethal phenotype, non-suppressible by Polr2B^S12

(Kim et al., 1994)

Other

Statement

Reference

Polr2A^Ubl, sno⁸ has lethal phenotype

(Lindsley and Zimm, 1992)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The recessive lethality of RpII215^Ubl is not rescued by one copy of RpII140^S1, RpII140^S2, RpII140^S5, RpII140^S6, RpII140^S8, RpII140^S7, RpII140^S11 or RpII140^S12.

(Kim et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

6328

y¹ ac^Hw-1 v¹ Polr2A^Ubl/FM7c

60337

y¹ ac^Hw-1 v¹ Polr2A^Ubl/FM7i

Kyoto

102043

y¹ ac^Hw-1 v¹ Polr2A^Ubl / FM7c

Notes on Origin

Discoverer

Comments

Causes the Ubx effect.

(Krasnoselskaya et al., 1998)

Gynandromorph analysis shows that the dominant Ubx-like phenotype, and the synergistic enhancement of Ubx by RpII215^Ubl requires that both a wild-type RpII215 and a RpII215^Ubl allele are present in the same cell.

(Mortin et al., 1985)

Degree of transformation of the haltere when heterozygous: RpII215^H1 < RpII215⁷ < RpII215^K2 < RpII215⁴ < RpII215^Ubl.

(Mortin et al., 1988)

Reduces the "Cbx" mesothorax phenotype of some Cbx alleles of Ubx. The reduction of the Cbx phenotype follows the order: Ubx^Cbx-Hm > Ubx^Cbx-2RM = Ubx^Cbx-2 = Ubx^Cbx-M1 > Ubx^Cbx-1RM = Ubx^Cbx-1 = Ubx^Cbx-3 = Ubx^Cbx-Twt.

(Gonzalez-Gaitan et al., 1990)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Polr2A^Ubl

RpII215^Ubl

(Popodi et al., 2010-)

Ubl

(Chen et al., 1993, Botas et al., 1988, Mortin et al., 1988, Voelker et al., 1985)

l(1)MGM179

Name Synonyms

Ultrabithorax-like

Secondary FlyBase IDs

References (13)

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