FlyBase Allele Report: Dmel\rl[9]

General Information

Symbol

Dmel\rl⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0014595

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

Also Known As

rl^EMS64, rl⁶⁴, EMS64

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Biggs et al., 1994)

loss of function allele

(Yamamoto et al., 1996)

Mutagen

ethyl methanesulfonate

(Biggs et al., 1994, Hilliker, 1976)

Progenitor genotype

Cytology

Description

Nonsense mutation at codon 353 predicted to result in a C-terminal truncation of 24 amino acids.

(Biggs et al., 1994)

Point mutation.

(Hilliker, 1976)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:1,120,676..1,120,676 [+]

Nucleotide change:

C1120676T

Amino acid change:

Reported amino acid change:

?353term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Biggs et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Inoue and Glover, 1998, Yamamoto et al., 1996)

visible

(Shamloula et al., 2002)

Phenotype Manifest In

eye

(Shamloula et al., 2002)

photoreceptor cell R7

(Yamamoto et al., 1996)

photoreceptor neuron

(Yamamoto et al., 1996)

Detailed Description

Statement

Reference

Mutants have a have a mild rough eye phenotype.

(Shamloula et al., 2002)

rl⁹/rl^10a heterozygotes die in the pupal stage. In contrast to wild type, there is very little increase in the mitotic index in either rl⁹/rl^10a or rl¹⁰/rl^10a mutants after colchicine treatment for 3 hrs, and over-condensation of the chromosomes is not seen.

(Inoue and Glover, 1998)

rl⁹/rl² flies lack the R7 photoreceptor cell and some outer photoreceptors. This phenotype is dominantly suppressed by aop^pok-1.

(Yamamoto et al., 1996)

Hemizygous larvae completely lack imaginal discs and die. Individuals die as pupae when heterozygous with rl¹ or rl⁶.

(Hilliker, 1976)

L3 larval lethal as hemizygote no imaginal discs pupal lethal when heterozygous with rl¹ or rl⁶ (Hilliker, 1976).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

rl⁹ is a non-enhancer of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

Suppressor of

Statement

Reference

rl[+]/rl⁹ is a suppressor of visible phenotype of rg^γ3

(Shamloula et al., 2002)

NOT Suppressor of

Statement

Reference

rl⁹ is a non-suppressor of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

rl[+]/rl⁹ is an enhancer of embryo | posterior phenotype of Dsor1^Su1, fs(1)M3¹⁹⁰¹

(Lim et al., 1999)

rl[+]/rl⁹ is an enhancer of embryonic abdominal segment 8 phenotype of Dsor1^Su1, fs(1)M3¹⁹⁰¹

(Lim et al., 1999)

Suppressor of

Statement

Reference

rl[+]/rl⁹ is a suppressor of eye phenotype of rg^γ3

(Shamloula et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from homozygous fs(1)M3¹⁹⁰¹ females fail to develop structures posterior to the seventh abdominal segment as well as anterior-most structures, including the head skeleton. The posterior defect is significantly suppressed if the female is also carrying one copy of Dsor1^Su1; an eighth abdominal segment is formed. This suppression is significantly reduced if the females are also carrying one copy of rl⁹.

(Lim et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Larval lethality rescued by rl^hs.PB construct.

(Biggs et al., 1994)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Hilliker.

Comments

Phenotypes of rl mutants fall into an allelic series: Df(2R)rl10a/rl¹ >= rl⁹/rl¹ > rl^la8/rl¹ > rl¹⁰/rl¹ > rl¹/rl¹.

(Diaz-Benjumea and Hafen, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

EMS64

(Dimitri, 1991, Hilliker, 1976)

l(2)EMS64

rl⁶⁴

(Inoue and Glover, 1998, Biggs et al., 1994)

rl⁹

rl^EMS64

(Shamloula et al., 2002, Lim et al., 1999, Sawamoto et al., 1999, Yamamoto et al., 1996, Diaz-Benjumea and Hafen, 1994)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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