FlyBase Allele Report: Dmel\Raf[1]

General Information

Symbol

Dmel\Raf¹

Species

D. melanogaster

Name

FlyBase ID

FBal0013769

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

Also Known As

raf^C110, D-raf^C110, Draf^C110, phl^C110

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Lee et al., 1996)

hypomorphic allele - genetic evidence

(Lim et al., 1997, Matsuo et al., 1997, Yamamoto et al., 1996, Hou et al., 1995, Brunner et al., 1994, Diaz-Benjumea and Hafen, 1994, Hata et al., 1994, Melnick et al., 1993, Perkins et al., 1992, Perrimon et al., 1985)

Mutagen

X ray

(Melnick et al., 1993)

Progenitor genotype

Cytology

Description

Amino acid replacement: R217L.

(Hata et al., 1994)

Amino acid replacement: R217L. Mutation in the CR1 domain.

(Lu et al., 1994)

Amino acid replacement: R217L. R217L lies in the CR1 protein domain.

(Melnick et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,341,535..2,341,535 [+]

Nucleotide change:

G2341535T

Amino acid change:

R174L | Raf-PA; R174L | Raf-PE

Reported amino acid change:

R217L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Melnick et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cone cell

(Matsuo et al., 1997)

eye

(Lu and Li, 1999, Zhang et al., 1999, Lim et al., 1997, Yamamoto et al., 1996, Hata et al., 1994, Perrimon et al., 1985)

eye | adult stage

(Lu et al., 1994)

eye photoreceptor cell

(Lim et al., 1997)

imaginal disc

(Hata et al., 1994)

imaginal disc | larval stage | somatic clone

(Lim et al., 1999)

ommatidium

(Lu and Li, 1999, Zhang et al., 1999, Yamamoto et al., 1996, Lu et al., 1994, Tsuda et al., 1993, Perrimon et al., 1985)

ommatidium | adult stage

(Lu et al., 1994)

photoreceptor cell R1

(Lu and Li, 1999, Lu et al., 1994)

photoreceptor cell R2

(Lu and Li, 1999, Lu et al., 1994)

photoreceptor cell R3

(Lu and Li, 1999, Lu et al., 1994)

photoreceptor cell R4

(Lu and Li, 1999, Lu et al., 1994)

photoreceptor cell R5

(Lu and Li, 1999, Lu et al., 1994)

photoreceptor cell R6

(Lu and Li, 1999, Lu et al., 1994)

photoreceptor cell R7

(Lu and Li, 1999, Zhang et al., 1999, Lim et al., 1997, Yamamoto et al., 1996)

thorax

(Tsuda et al., 1993)

wing vein

(Hata et al., 1994)

Detailed Description

Statement

Reference

Embryos derived from mutant germ line clones show normal posterior pattern elements. The extent to which posterior pattern elements are formed directly reflects the magnitude of tor signal. Thus mutants derived from germ line clones can be ordered with respect to increasing posterior abnormalities thus: phl¹ (normal posterior patterning) - csw⁶ - csw⁶,phl¹ - csw⁵,phl^PB26 (deletion of A8).

(Ghiglione et al., 1999)

Clonal analysis indicates that the rate of proliferation in imaginal discs is reduced by approximately 40% in homozygous clones compared to wild-type.

(Lim et al., 1999)

Hemizygous pharate adult males have rough eyes due to the formation of disorganised ommatidial arrays and missing outer and inner photoreceptor cells.

(Lu and Li, 1999)

phl¹/Y pharate adults have rough eyes and the R7 cell is missing in 88% of ommatidia.

(Zhang et al., 1999)

Homozygotes and hemizygotes die during or soon after eclosion, and have a rough eye phenotype mainly due to loss of inner R7 and some outer photoreceptor cells.

(Lim et al., 1997)

Decrease in the number of cone cells in most ommatidia.

(Matsuo et al., 1997)

Daily heat shocks of P{hs-Draf^wt} can rescue phl¹ inviability. Discontinuation of heat shocks reveals no change in initiation or completion of border cell migration.

(Lee et al., 1996)

Hemizygous escapers eclose as adults with rough eyes, with most ommatidia lacking the R7 cell. The lifespan of hemizygous flies is reduced after eclosion compared to wild-type flies.

(Yamamoto et al., 1996)

Homozygotes show no apparent retardation in growth of the imaginal discs but mitotic clones reveal a significant decrease in growth rate. Flies have a rough eye phenotype and a mild wing vein phenotype and die soon after emergence.

(Hata et al., 1994)

Hemizygous males display defects in cell proliferation causing small eyes, severely disorganised ommatidia due to missing outer photoreceptor cells and fusion of ommatidial units.

(Lu et al., 1994)

Class 3 allele: no maternal effect. Die during pupariation or eclosion, with rough eye phenotype. Ommatidia show variable absences of photoreceptor and non-photoreceptor cells.

(Melnick et al., 1993)

Defects of muscle or nervous system of the thorax. Rough eye phenotype due to reduced numbers of ommatidia. Structures posterior to the seventh abdominal segment are missing in the embryos derived from homozygous germ line clones.

(Tsuda et al., 1993)

tll expression severely reduced posteriorly.

(Perkins et al., 1992)

Lethal phase in homozygotes is at the pupal-adult boundary. There is no effect of temperature on the lethal phase. The number of ommatidia in emerging males is reduced by 30% from wild type. Weak maternal effect: germ line clone analysis reveals no maternal effect on the lethal phase, though a few embryos failed to hatch. The embryos show no cuticular abnormality.

(Perrimon et al., 1985)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Raf¹ has lethal phenotype, suppressible by Src42A^Su(Raf)1-1

(Zhang and Lu, 2000, Lu and Li, 1999)

Raf¹ has visible | recessive phenotype, suppressible by Src42A^Su(Raf)1-1

(Lu and Li, 1999)

Raf¹ has lethal phenotype, suppressible by Src42A^7-4

(Lu and Li, 1999)

Raf¹ has visible | recessive phenotype, suppressible by Df(2R)nap8

(Lu and Li, 1999)

Raf¹ has lethal | recessive phenotype, suppressible by Src42A^Su(Raf)1-1

(Zhang et al., 1999)

Raf¹ has short lived phenotype, suppressible by aop^pok-1/aop[+]

(Yamamoto et al., 1996)

NOT suppressed by

Statement

Reference

Raf¹ has lethal phenotype, non-suppressible by cnk^sag13L/Src42A^Su(Raf)1-1

(Zhang and Lu, 2000)

Raf¹ has lethal phenotype, non-suppressible by Src42A^Su(Raf)1-1/cnk^sag32-3

(Zhang and Lu, 2000)

Raf¹ has lethal phenotype, non-suppressible by Src42A^18-2

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Src42A^9-4

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Src42A^k10108

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Src42A^k10115

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Df(2R)nap8

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Df(2R)nap9

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Df(2R)nap1

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by In(2R)bw^VDe2LCy^R

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Src42A^1.15

(Lu and Li, 1999)

Raf¹ has lethal phenotype, non-suppressible by Src42A^15-1

(Lu and Li, 1999)

Raf¹ has lethal | recessive phenotype, non-suppressible by Df(2R)nap9

(Zhang et al., 1999)

Enhancer of

Statement

Reference

Raf¹ is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

NOT Enhancer of

Statement

Reference

Raf¹ is a non-enhancer of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

Suppressor of

Statement

Reference

Raf¹ is a suppressor of visible phenotype of Scer\GAL4^c684, if^m8.UAS

(Baker et al., 2002)

Raf¹ is a suppressor of visible phenotype of Scer\GAL4^sca-109-68, ato^UAS.cJa

(White and Jarman, 2000)

Raf[+]/Raf¹ is a suppressor of short lived phenotype of aop^pok-1

(Yamamoto et al., 1996)

NOT Suppressor of

Statement

Reference

Raf¹ is a non-suppressor of visible phenotype of if^m8.αTub84B

(Baker et al., 2002)

Raf¹ is a non-suppressor of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

Other

Statement

Reference

Raf¹, Src42A^7-4 has viable phenotype

(Lu and Li, 1999)

Raf¹, Src42A^Su(Raf)1-1 has viable phenotype

(Lu and Li, 1999, Zhang et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Raf¹ has phenotype, suppressible by rl^Su14

(Lim et al., 1999)

Raf¹ has eye phenotype, suppressible by Src42A^Su(Raf)1-1

(Lu and Li, 1999, Lu et al., 1994)

Raf¹ has ommatidium phenotype, suppressible by Src42A^Su(Raf)1-1

(Lu and Li, 1999, Zhang et al., 1999)

Raf¹ has photoreceptor cell R7 phenotype, suppressible by Src42A^Su(Raf)1-1

(Lu and Li, 1999, Zhang et al., 1999)

Raf¹ has eye phenotype, suppressible by Df(2R)nap8

(Lu and Li, 1999)

Raf¹ has ommatidium phenotype, suppressible by Df(2R)nap8

(Lu and Li, 1999)

Raf¹ has photoreceptor cell R7 phenotype, suppressible by Df(2R)nap8

(Lu and Li, 1999)

Raf¹ has phenotype, suppressible by Dsor1^Su12

(Lim et al., 1997)

Raf¹ has phenotype, suppressible by Dsor1^Su18

(Lim et al., 1997)

Raf¹ has phenotype, suppressible by Dsor1^Su1

(Lim et al., 1997, Tsuda et al., 1993)

Raf¹ has phenotype, suppressible by Dsor1^Su24

(Lim et al., 1997)

Raf¹ has phenotype, suppressible by Dsor1^Su4

(Lim et al., 1997)

Raf¹ has phenotype, suppressible by Dsor1^Su5

(Lim et al., 1997)

Raf¹ has phenotype, suppressible by Dsor1^Su6

(Lim et al., 1997)

Raf¹ has phenotype, suppressible by Dsor1^Su7

(Lim et al., 1997)

Raf¹ has photoreceptor cell R7 phenotype, suppressible by aop^pok-1/aop[+]

(Yamamoto et al., 1996)

Raf¹ has eye phenotype, suppressible by Dsor1^Su34B

(Lu et al., 1994)

Raf¹ has eye phenotype, suppressible by Su(phl)43B¹

(Lu et al., 1994)

Raf¹ has eye phenotype, suppressible by Su(phl)4¹

(Lu et al., 1994)

Raf¹ has eye phenotype, suppressible by Su(phl)6¹

(Lu et al., 1994)

Raf¹ has eye phenotype, suppressible by Su(phl)7¹

(Lu et al., 1994)

Raf¹ has eye phenotype, suppressible by Su(phl)9¹

(Lu et al., 1994)

Enhancer of

Statement

Reference

Raf¹ is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Suppressor of

Statement

Reference

Raf¹ is a suppressor of wing phenotype of Scer\GAL4^c684, if^m8.UAS

(Baker et al., 2002)

Raf¹ is a suppressor of eye phenotype of Scer\GAL4^sca-109-68, ato^UAS.cJa

(White and Jarman, 2000)

Raf¹ is a suppressor of photoreceptor cell R1 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Raf¹ is a suppressor of photoreceptor cell R2 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Raf¹ is a suppressor of photoreceptor cell R3 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Raf¹ is a suppressor of photoreceptor cell R4 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Raf¹ is a suppressor of photoreceptor cell R5 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Raf¹ is a suppressor of photoreceptor cell R6 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Raf¹ is a suppressor of phenotype of svp^1.sev

(Begemann et al., 1995)

Raf¹ is a suppressor of phenotype of svp^2.sev

(Begemann et al., 1995)

NOT Suppressor of

Statement

Reference

Raf¹ is a non-suppressor of wing phenotype of if^m8.αTub84B

(Baker et al., 2002)

Raf¹ is a non-suppressor of photoreceptor cell R7 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Other

Statement

Reference

Raf¹, csw⁶ has embryonic epidermis | posterior phenotype

(Ghiglione et al., 1999)

Raf¹, rl[+]/rl^Sem has photoreceptor neuron phenotype

(Brunner et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

rl^Su14 and rl^Su23 fully suppress the phl¹ phenotype in terms of viability and the morphologies of the eye and wing veins. The imaginal disc proliferation defect is suppressed by rl^Su23/+.

(Lim et al., 1999)

The lethality of phl¹ is suppressed by Src42A^Su(phl)1-1 and Src42A^7-4. The lethality of phl¹ is not suppressed by Src42A^15-1, Src42A^18-2, Src42A^k10115, Src42A^k10108, Src42A^1.15, Src42A^9-4, Df(2R)nap8, Df(2R)nap9, Df(2R)nap1 or In(2R)bw^VDe2LCy^R. All the aspects of the hemizygous rough eye phenotype are suppressed by Df(2R)nap8, although viability is not restored in these flies. The rough eye phenotype is also suppressed by Src42A^Su(phl)1-1.

(Lu and Li, 1999)

The phl¹ phenotype is suppressed by Src42A^Su(phl)1-1; phl¹/Y flies die (as late pupae or a few hours following eclosion), whereas phl¹/Y ; Src42A^Su(phl)1-1/+ flies are viable and fertile. phl¹/Y ; Df(2R)nap9/+ flies are not viable. Rescued phl¹/Y ; Src42A^Su(phl)1-1/+ flies have rough eyes and the R7 cell is missing in 36% of ommatidia.

(Zhang et al., 1999)

phl¹ significantly suppresses the formation of extra outer photoreceptors caused by B-H1^sev.PH, although it does not affect the loss of R7 cell phenotype caused by B-H1^sev.PH.

(Hayashi et al., 1998)

The phenotype is dominantly suppressed by Dsor1^Su1, Dsor1^Su4, Dsor1^Su5, Dsor1^Su6, Dsor1^Su7, Dsor1^Su12, Dsor1^Su18 and Dsor1^Su24.

(Lim et al., 1997)

R7 is rescued in phl¹ hemizygotes that are also heterozygous for aop^pok-1. The longevity of aop^pok-1 homozygotes is prolonged in a phl¹ heterozygous background, although the longevity of phl¹ hemizygotes is not affected by the presence of aop^pok-1.

(Yamamoto et al., 1996)

Weak suppressor of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

(Begemann et al., 1995)

Reduced viability is dominantly suppressed by rl^Sem. Surviving flies hemizygous for phl¹ have a reduced number of photoreceptors, but phl¹ homozygotes also mutant for rl^Sem have wild type eyes.

(Brunner et al., 1994)

Homozygous females carrying one copy of Su(phl)6, Su(phl)43B, Su(phl)7 produce a significant proportion of eggs with two normal dorsal appendages. One copy of Src42A produces variable eggs with normal or fused dorsal appendages. Su(phl)9, Su(phl)4 and Dsor1^Su34B produce few eggs with dorsal appendages.

(Lu et al., 1994)

Maternal, not paternal, contribution of Dsor1^Su1 partially rescues the defects seen in embryos derived from homozygous germ line clones.

(Tsuda et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

(Lu et al., 1994)

(Lu et al., 1994)

(Lu et al., 1994)

(Lu et al., 1994)

Fails to complement

Raf⁷

(Perrimon et al., 1985)

Raf⁶

(Perrimon et al., 1985)

Raf⁵

(Perrimon et al., 1985)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lefevre.

Comments

Weak phl allele.

(Tsuda et al., 1993)

Allele designation of hypomorph based on observation that homozygotes die at pupal-adult interface as but heterozygotes with Df(1)64c18 die as pupae. Isolated in screen described in Perrimon et al., Dev. Biol. 110: 480.

(Melnick et al., 1993)

The R217L amino acid replacement abolishes any interaction between the phl and Ras85D products.

(Hou et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

C110

(Kramers et al., 1983)

D-Raf^C110

(Hou et al., 1995)

D-raf¹

(Sawamoto et al., 1999)

D-raf^C110

(Morey et al., 2001, Adachi-Yamada et al., 1999, Ghiglione et al., 1999, Lim et al., 1999, Lim et al., 1997, Nishida et al., 1996, Perrimon et al., 1995, Hata et al., 1994, Lu et al., 1994, Melnick et al., 1993, Tsuda et al., 1993)

Draf^C110

(Baker et al., 2002, Li et al., 2002, Li et al., 1998)

Raf¹

Raf^C110

(Zhang and Lu, 2000, Lu and Li, 1999, Zhang et al., 1999)

l(1)C110

l(1)raf¹

(Nishida, 1990)

phl¹

phl^C110

(Settle et al., 2003, White and Jarman, 2000, Perrimon et al., 1985)

raf1^c110

(Yamamoto et al., 1996)

raf¹

(Hayashi et al., 1998)

raf^C110

(Zhu et al., 2005, Staehling-Hampton et al., 1999, Matsuo et al., 1997, Begemann et al., 1995, Brunner et al., 1994, Diaz-Benjumea and Hafen, 1994, Perkins et al., 1992)

raf^c110

(Boulton et al., 2000, Matsuo et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (38)

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