FlyBase Allele Report: Dmel\otu[17]

General Information

Symbol

Dmel\otu¹⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0013364

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Storto and King, 1987, King et al., 1986)

Progenitor genotype

Cytology

Description

Deletion of part of the coding region (Mulligan et al., Mol Cell Biol 8: 1481-1488).

(Geyer et al., 1993)

2 kb deletion

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female

(Tirronen et al., 1993)

female sterile

(Sass et al., 1995, Spradling et al., 1994)

female sterile | recessive

(Geyer et al., 1993, King et al., 1986)

neoplasia | dominant | oogenesis

(Primus et al., 2019)

Phenotype Manifest In

germarium

(Primus et al., 2019)

ovariole

(Pauli et al., 1993, Storto and King, 1987, King et al., 1986)

ovary

(Primus et al., 2019, Geyer et al., 1993)

Detailed Description

Statement

Reference

otu¹⁷ heterozygotes show a rare and mild ovary 'germline tumor' phenotype; homozygotes lack germline cells.

(Primus et al., 2019)

Ovaries contain tumorous egg cysts.

(Geyer et al., 1993)

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males.

(Tirronen et al., 1993)

92% of homozygous ovarioles have quiescent oogonia, and 8% have tumorous oogonia.

(Storto and King, 1987)

Quiescent allele; homozygous and hemizygous ovarioles usually contain vitellaria that lack developing follicles, 8% or less of the oogonia are tumour-generating.

(King et al., 1986)

quiescent; ovarioles lack germ cells

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

otu¹⁷ has neoplasia | oogenesis | dominant phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/CG15930^UAS.cPa

(Primus et al., 2019)

Other

Statement

Reference

Sxl^f4/Sxl^f2, otu¹⁷ has female sterile | dominant phenotype

(Sun and Cline, 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

otu¹⁷ has germarium phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/CG15930^UAS.cPa

(Primus et al., 2019)

otu¹⁷ has ovary phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/CG15930^UAS.cPa

(Primus et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

otu¹⁷ dominantly suppresses the ability of Wolbachia to suppress Sxl^f4 defects in oogenesis.

Almost no Sxl^f2/Sxl^f4 females make eggs if they also carry otu¹⁷/+. Most egg chambers in these females are tumorous.

The phenotype of a reduction in recombination across the y-sn interval on the X chromosome that is seen in Sxl^M1,f3/Sxl^fs3 females is enhanced by otu¹⁷/+ ; there is a further reduction in map distance in the double mutants. In addition, the rate of X chromosome nondisjunction is increased eightfold.

Sxl^M1,f3, otu¹⁷ double heterozygotes show reduced recombination across the y-sn interval on the X chromosome compared to wild type, with the reduction being stronger than the weak reduction seen in Sxl^M1,f3 single heterozygotes.

(Sun and Cline, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

otu^SuWs

(Sun and Cline, 2009)

Rescued by

otu¹⁷ is rescued by otu^+t5.4

(Comer et al., 1992)

Comments

Rescued by otu^+t5.4 : fertility, normal ovarian development and normal 3.2kb message expressed in ovaries is restored.

(Comer et al., 1992)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38431

y¹ w^* otu¹⁷/FM7a; P{ovo-lacZ.Δap}S1

Notes on Origin

Discoverer

Digan.

Comments

Mutation reduces the ability of Sxl^cF1.otu to induce female Sxl⁺ RNA splicing in the male germline: number of autoregulatory cysts in testes is much reduced.

(Hager and Cline, 1997)

Quiescent (QUI) class allele. The ovarian phenotype of flies transheterozygous for otu¹⁷ and a number of other otu mutations has been analysed.

(Storto and King, 1987)

One of QUI class of alleles.

(Comer et al., 1992)

Likely to be a null mutation.

(Geyer et al., 1993)

Shows a dominant interaction with ovo^D2 and ovo^D3 : more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles.

(Pauli et al., 1993)

QUI class of otu allele. P{otu-98} fails to restore detectable levels of fertility, P{otu-104} completely restores fertility.

(Sass et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

1401

(Mohler and Carroll, 1984)

fs(1)1401

(Spradling et al., 1994)

fs1401

(King et al., 1986)

otu¹⁷

(Primus et al., 2019, Sun and Cline, 2009)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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