FlyBase Allele Report: Dmel\Nrg[l3]

General Information

Symbol

Dmel\Nrg^l3

Species

D. melanogaster

Name

FlyBase ID

FBal0013168

Feature type

allele

Associated gene

Dmel\Nrg

Associated Insertion(s)

Carried in Construct

Also Known As

Nrg³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

Progenitor genotype

Cytology

Description

Amino acid replacement: G313D.

(Yamamoto et al., 2006)

Amino acid replacement: V318M.

(Yamamoto et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,545,584..8,545,584 [+]

Nucleotide change:

G8545584A

Amino acid change:

Reported amino acid change:

G313D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Yamamoto et al., 2006)

point_mutation

X:8,545,601..8,545,601 [+]

Nucleotide change:

G8545601A

Amino acid change:

Reported amino acid change:

V318M

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Yamamoto et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Nagaraj et al., 2009, Kristiansen et al., 2005)

abnormal neuroanatomy | heat sensitive

(Godenschwege, 2006, Godenschwege et al., 2006, Yamamoto, 2006)

abnormal neurophysiology | heat sensitive

(Godenschwege et al., 2006)

Phenotype Manifest In

dorsal multidendritic neuron ddaD & axon | conditional ts

(Yamamoto, 2006)

dorsal multidendritic neuron ddaD & dendritic tree | conditional ts

(Yamamoto, 2006)

dorsal multidendritic neuron ddaE & axon | conditional ts

(Yamamoto, 2006)

dorsal multidendritic neuron ddaE & dendritic tree | conditional ts

(Yamamoto, 2006)

giant fiber neuron | heat sensitive

(Godenschwege, 2006, Godenschwege et al., 2006)

larval dorsal multidendritic neuron ddaD | heat sensitive

(Yamamoto, 2006)

larval dorsal multidendritic neuron ddaE

(Yamamoto, 2006)

mechanosensory neuron

(Nagaraj et al., 2009, Kristiansen et al., 2005)

ocellar nerve

(Nagaraj et al., 2009, Kristiansen et al., 2005)

presynapse | heat sensitive

(Godenschwege, 2006)

synapse | heat sensitive

(Godenschwege, 2006, Godenschwege et al., 2006)

wing, with Nrg^167.UAS, Scer\GAL4^MS1075

(Islam et al., 2004)

wing, with Nrg^GPI.UAS, Scer\GAL4^MS1075

(Islam et al., 2004)

Detailed Description

Statement

Reference

Nrg^l3 mutant flies exhibit a stalling phenotype and, less frequently, in misguidance defects of ocellar and orbital bristle mechanosensory (BM) axons. Sometimes only one or a subset of axons seems to stall within a nerve, although other axons extend normally. In a few cases, BM axons separate from the epidermis without connecting with the orbital nerve, or project backwards or to an ectopic place. In a few cases, BM axons separate from the epidermis without connecting with the orbital nerve, or project backwards or to an ectopic place.

(Nagaraj et al., 2009)

A temporal loss of Nrg, using the temperature-sensitive Nrg^l3 mutant for 12.5% or 25% of pupal development during synapse formation and well as synapse maturation is able to disrupt the giant synapse anatomically and physiologically. In 50% of cases, the complete synaptic terminal is lacking when the temporal loss of function is induced during synapse formation.

(Godenschwege et al., 2006)

When Nrg^l3 mutants are exposed to nonpermissive temperatures during synapse formation (20-40% of pupal development) or during synaptic maturation (approximately 40-75% of pupal development), presynaptic terminals are absent or abnormally thin. When adults are exposed to nonpermissive temperatures, large vesicles in the giant fiber terminals are observed.

(Godenschwege, 2006)

Temperature shift experiments with Nrg^l3 embryos show that Nrg function is necessary by 15 hours AEL for morphogenesis of da neurons and for correct axon extension and dendrite arborization.

(Yamamoto, 2006)

Overexpression of either Nrg^167.Scer\UAS or Nrg^GPI.Scer\UAS in wing sensory neurons and two wing epithelial layers, mediated by Scer\GAL4^MS1075, in an Nrg^l3 background causes the same defective wing phenotypes that occur when these transgenes are expressed in a wild-type background.

(Islam et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nrg^l3 has abnormal neuroanatomy phenotype, enhanceable by Fas2[+]/Fas2^e76

(Kristiansen et al., 2005)

Suppressed by

Statement

Reference

Nrg^l3 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^MS1075/Hsap\L1CAM^RSLE-.UAS

(Nagaraj et al., 2009, Kristiansen et al., 2005)

Nrg^l3 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^MS1075/Mmus\L1cam^RSLE+.UAS

(Kristiansen et al., 2005)

Nrg^l3 has abnormal neuroanatomy phenotype, suppressible by Rnor\Nrcam^UAS.cKa/Scer\GAL4^MS1075

(Kristiansen et al., 2005)

Nrg^l3 has abnormal neuroanatomy | temperature conditional phenotype, suppressible by Hsap\NCAM1^140.UAS/Scer\GAL4^MS1075

(Kristiansen et al., 2005)

NOT suppressed by

Statement

Reference

Nrg^l3 has abnormal neuroanatomy phenotype, non-suppressible by Hsap\L1CAM^H1.UAS/Scer\GAL4^MS1075

(Nagaraj et al., 2009)

Nrg^l3 has abnormal neuroanatomy phenotype, non-suppressible by Hsap\L1CAM^H38.UAS/Scer\GAL4^MS1075

(Nagaraj et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nrg^l3 has ocellar nerve phenotype, enhanceable by Fas2[+]/Fas2^e76

(Kristiansen et al., 2005)

NOT Enhanced by

Statement

Reference

Nrg^l3 has mechanosensory neuron phenotype, non-enhanceable by Fas2[+]/Fas2^e76

(Kristiansen et al., 2005)

Suppressed by

Statement

Reference

Nrg^l3 has ocellar nerve phenotype, suppressible by Scer\GAL4^MS1075/Hsap\L1CAM^RSLE-.UAS

(Nagaraj et al., 2009, Kristiansen et al., 2005)

Nrg^l3 has mechanosensory neuron phenotype, suppressible by Scer\GAL4^MS1075/Hsap\L1CAM^RSLE-.UAS

(Nagaraj et al., 2009)

Nrg^l3 has ocellar nerve phenotype, suppressible by Scer\GAL4^MS1075/Mmus\L1cam^RSLE+.UAS

(Kristiansen et al., 2005)

Nrg^l3 has ocellar nerve phenotype, suppressible by Rnor\Nrcam^UAS.cKa/Scer\GAL4^MS1075

(Kristiansen et al., 2005)

Nrg^l3 has mechanosensory neuron phenotype, suppressible | partially by Rnor\Nrcam^UAS.cKa/Scer\GAL4^MS1075

(Kristiansen et al., 2005)

Nrg^l3 has ocellar nerve | temperature conditional phenotype, suppressible by Hsap\NCAM1^140.UAS/Scer\GAL4^MS1075

(Kristiansen et al., 2005)

Nrg^l3 has mechanosensory neuron | temperature conditional phenotype, suppressible by Hsap\NCAM1^140.UAS/Scer\GAL4^MS1075

(Kristiansen et al., 2005)

Nrg^l3 has mechanosensory neuron phenotype, suppressible | partially by Scer\GAL4^MS1075/Hsap\L1CAM^RSLE-.UAS

(Kristiansen et al., 2005)

Nrg^GPI.UAS, Nrg^l3, Scer\GAL4^MS1075 has wing phenotype, suppressible by Egfr[+]/Egfr^t1

(Islam et al., 2004)

Nrg^167.UAS, Nrg^l3, Scer\GAL4^MS1075 has wing phenotype, suppressible by Egfr[+]/Egfr^t1

(Islam et al., 2004)

NOT suppressed by

Statement

Reference

Nrg^l3 has mechanosensory neuron phenotype, non-suppressible by Hsap\L1CAM^H1.UAS/Scer\GAL4^MS1075

(Nagaraj et al., 2009)

Nrg^l3 has ocellar nerve phenotype, non-suppressible by Hsap\L1CAM^H1.UAS/Scer\GAL4^MS1075

(Nagaraj et al., 2009)

Nrg^l3 has mechanosensory neuron phenotype, non-suppressible by Hsap\L1CAM^H38.UAS/Scer\GAL4^MS1075

(Nagaraj et al., 2009)

Nrg^l3 has ocellar nerve phenotype, non-suppressible by Hsap\L1CAM^H38.UAS/Scer\GAL4^MS1075

(Nagaraj et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

A heterozygous Fas2^e76 background acts as a dominant enhancer of the Nrg^l3 defects in OP axons at the restrictive temperature. The axonal guidance defects of BM axons in Nrg^l3 are not affected.

(Kristiansen et al., 2005)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\L1CAM^{RSLE-.Scer\UAS} under the control of Scer\GAL4^MS1075 suppresses the bristle mechanosensory (BM) axonal defects seen in Nrg^l3 mutant flies.

Expression of Hsap\L1CAM^H1.Scer\UAS under the control of Scer\GAL4^MS1075 does not suppress the bristle mechanosensory (BM) axonal defects seen in Nrg^l3 mutant flies.

Expression of Hsap\L1CAM^H38.Scer\UAS under the control of Scer\GAL4^MS1075 does not suppress the bristle mechanosensory (BM) axonal defects seen in Nrg^l3 mutant flies.

(Nagaraj et al., 2009)

Expression of Hsap\L1CAM^{RSLE-.Scer\UAS} under the control of Scer\GAL4^MS1075 in Nrg^l3 mutant individuals rescues the axonal defects in OP axons, along with slightly reducing the level of BM axon defects.

Expression of Mmus\L1cam^{RSLE+.Scer\UAS} under the control of Scer\GAL4^MS1075 in Nrg^l3 mutant individuals rescues the axonal defects in OP axons.

Expression of Rnor\Nrcam^Scer\UAS.cKa under the control of Scer\GAL4^MS1075 in Nrg^l3 mutant individuals rescues the axonal defects in OP axons, along with slightly reducing the level of BM axon defects.

Expression of Hsap\NCAM1^140.Scer\UAS in a Nrg^l3 background, under the control of Scer\GAL4^MS1075 suppresses the Nrg^l3 phenotype at the restrictive temperature, specifically OP axon alterations and BM axon guidance defects.

(Kristiansen et al., 2005)

Complementation and Rescue Data

Rescued by

Nrg^l3 is rescued by Scer\GAL4^MS1075/Nrg^180.UAS

(Kristiansen et al., 2005)

Not rescued by

Nrg^l3 is not rescued by Nrg^167.UAS/Scer\GAL4^MS1075

(Kristiansen et al., 2005)

Comments

Scer\GAL4^MS1075-driven expression of neural-Nrg^180.Scer\UAS in Nrg^l3 mutant animals rescues both the pentrance and the expressivity of the OP and BM axon misguidance defects.

Scer\GAL4^MS1075-driven expression of Nrg^167.Scer\UAS in Nrg^l3 mutant animals shows a synergistic increase in the expressivity of OP axon alterations (i.e. axonal alterations are much more severe in those individuals that display the phenotype).

(Kristiansen et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lefevre.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Nrg³

(Godenschwege et al., 2006, Islam et al., 2004)

Nrg^l3

l(1)HF336

nrg³

(Nagaraj et al., 2009, Godenschwege, 2006, Yamamoto, 2006, Yamamoto et al., 2006, Kristiansen et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (8)

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