FlyBase Allele Report: Dmel\norpA[P12]

General Information

Symbol

Dmel\norpA^P12

Species

D. melanogaster

Name

FlyBase ID

FBal0013126

Feature type

allele

Associated gene

Dmel\norpA

Associated Insertion(s)

Carried in Construct

Also Known As

norpA³³

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Pearn et al., 1996)

Mutagen

ethyl methanesulfonate

(Pearn et al., 1996)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C1370T (nucleotide numbering as in FBrf0047660), producing a premature termination codon.

(Pearn et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:4,357,358..4,357,358 [+]

Nucleotide change:

C4357358T

Reported nucleotide change:

C1370T

Amino acid change:

Reported amino acid change:

?240term

(Pearn et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage | recessive

(Busto et al., 1999)

abnormal neuroanatomy | conditional

(Chen et al., 2012)

abnormal neurophysiology

(Hardie et al., 2002)

Phenotype Manifest In

eye photoreceptor cell | conditional

(Chen et al., 2012)

photoreceptor neuron

(Hardie et al., 2002)

Detailed Description

Statement

Reference

Most photoreceptor cells are degenerated in mutant adults after 6 days under light conditions.

(Chen et al., 2012)

The amplitude of quantum bumps in mutant photoreceptors is smaller than wild type (when ATP is present in the intracellular solution during recordings).

(Hardie et al., 2002)

Wild-type larvae reduce their path lengths and show increased head swinging when exposed to light. This response is abolished in norpA³³ larvae. Wild-type larvae show a greater change in direction when lights are turned on or off (light (L) to dark (D), or D to L transition) than in the absence of a light transition (D to D). The amplitude of change of direction is greater for the D to L than for the L to D transition. This light-induced difference in the amplitude of change of direction for L to D and D to L transitions is abolished in norpA³³ larvae. The difference between the change of direction at the L to D transition and in the absence of a light transition (D to D) is not affected in norpA³³ larvae.

(Busto et al., 1999)

The DHP-sensitive current is reduced. Application of TPA (phorbol 12-myrisatae 13-acetate) and PDD (phorbol 12,13-didecanoate), activators of PKC, rescues the current in mutant fibres without significantly affecting the normal current. 4αPDD (4α-phorbol 12,13-didecanoate), an inactive analog of PDD, does not affect the normal or mutant current. BIM (bisindolylmaleimide), an inhibitor of PKC, reduces the current in normal fibres without affecting the mutant current. DOG (sn-1,2-dioctanoyl-glycarol), analog of diacylglycerol increases the current in the mutant fibres.

(Gu and Singh, 1997)

The electroretinogram (ERG) of norpA³³ flies shows reduced amplitude and slower response kinetics compared to that of wild-type flies.

(Pearn et al., 1996)

The amplitude response to vapours of all odorants tested in the maxillary palp is not significantly different from wild type. The antennal response is also unaffected.

(Riesgo-Escovar et al., 1995)

intermediate ERG

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

norpA^P12 has abnormal neuroanatomy | conditional phenotype, suppressible by ktub^115-4

(Chen et al., 2012)

norpA^P12 has abnormal neurophysiology phenotype, suppressible by rdgA¹

(Hardie et al., 2002)

Suppressor of

Statement

Reference

norpA^P12 is a suppressor of abnormal neuroanatomy | conditional phenotype of ktub^115-4

(Chen et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

norpA^P12 has eye photoreceptor cell | conditional phenotype, suppressible by ktub^115-4

(Chen et al., 2012)

norpA^P12 has photoreceptor neuron phenotype, suppressible by rdgA¹

(Hardie et al., 2002)

Suppressor of

Statement

Reference

norpA^P12 is a suppressor of eye photoreceptor cell | conditional phenotype of ktub^115-4

(Chen et al., 2012)

norpA^P12 is a suppressor of rhabdomere phenotype of rdgA¹

(Georgiev et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

norpA³³ ; ktub^115-4 double mutants have almost normal photoreceptor organisation after 6 days in constant light.

(Chen et al., 2012)

norpA³³ almost completely suppresses the retinal degeneration seen in rdgA¹ flies.

(Georgiev et al., 2005)

The small amplitude of quantum bumps in norpA³³ photoreceptors (seen when ATP is present in the intracellular solution during recordings) is increased in size to near wild-type levels if the flies are also mutant for rdgA¹.

(Hardie et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9047

w^* norpA^P12

Notes on Origin

Discoverer

Pak.

Comments

norpA RNA and protein expression, and phospholipase C activity have been studied in norpA³³ flies.

(Zhu et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

norpA³³

(Jiang et al., 2020, Chen et al., 2012)

norpA^P12

(Georgiev et al., 2005, Caldwell et al., 2003, Hardie et al., 2003, Hardie et al., 2002, Cook et al., 2000, Busto et al., 1999, Gu and Singh, 1997, Pearn et al., 1996, Riesgo-Escovar et al., 1995, McKay et al., 1994, Zhu et al., 1993, Pearn et al., 1991)

norpA^p33

(Sun et al., 2020)

x12

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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