FlyBase Allele Report: Dmel\mam[8]

General Information

Symbol

Dmel\mam⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0012016

Feature type

allele

Associated gene

Dmel\mam

Associated Insertion(s)

Carried in Construct

Also Known As

mam^IJ113

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yedvobnick et al., 1988, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Schimke immuno-osseous dysplasia

modeled by Marcal1^UAS.cBa

(Morimoto et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage 16

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 16 (with mam^ΔC)

(Zhang et al., 2011)

lethal - all die during embryonic stage | neurogenic phenotype | recessive

(Lehmann et al., 1983)

some die during embryonic stage | recessive

(Lehmann et al., 1983)

Phenotype Manifest In

Detailed Description

Statement

Reference

Midline glia are present in mam⁸ mutant stage 16 embryos but appear disorganised compared to wild type.

Midline glia are present in mam⁸/mam^ΔC mutant stage 16 embryos but appear disorganised in comparison with wild type.

(Zhang et al., 2011)

Homozygous mutant follicle stem cell clones are present at a much lower frequency than control clones at 7 days (30% vs 73%) and 14 days (8% vs 57%) after clone induction.

Ovarioles that include mutant follicle cells show frequent egg chamber fusions.

(Vied and Kalderon, 2009)

Homozygous clones in the developing eye have no effect on eye development.

(Li and Baker, 2001)

Embryonic CNS exhibits severe disturbances and larval exhibit severe cuticle loss.

(Schmid et al., 1996)

Embryos exhibit fused muscles in patterned arrangements, particularly in the more dorsal regions of the embryo. The birefringent is clearly correlated with the expansion of the CNS and PNS, and the loss of epidermis and the degree to which myoblast fusion occurs. Where myoblast fusion fails conspicuous clusters of mesodermal cells are formed and if epidermal territories are expanded cells in these clusters may be recruited to fusion.

(Bate et al., 1993)

Intermediate neurogenic phenotype.

(Lieber et al., 1993)

Homozygous embryos have a greatly enlarged ventral nerve cord.

(Kovalick and Beckingham, 1992)

mam⁸ shows weak neural hypertrophy, a 2-5 fold increase in nau expressing cells per cluster relative to wild type.

(Corbin et al., 1991)

The anterior end of the embryo is filled with neural cells and the ventral nerve cord is expanded.

(Yedvobnick et al., 1988)

Intermediate embryonic neurogenic phenotype.

(Lehmann et al., 1983)

stronger allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

mam⁸ has follicle stem cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/N^intra.GS.UAS

(Vied and Kalderon, 2009)

mam⁸ has follicle stem cell | somatic clone phenotype, non-suppressible by Su(H)^VP16.UAS.cSa/Scer\GAL4^Tub.PU

(Vied and Kalderon, 2009)

Suppressor of

Statement

Reference

mam⁸/mam[+] is a suppressor of phenotype of hh^hs.PI

(Vied and Kalderon, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The phenotype of excess follicle stem cell progeny in between egg chambers that is caused by expression of hh^hs.PI using heat shock twice daily for 3 days followed by a 3 day "chase" is dominantly suppressed by mam⁸, such that the number of inappropriate cells between egg chambers is strongly reduced.

mam⁸ prevents the duplication of follicle stem cells that is normally seen in ptc^S2 follicle stem cell clones.

Expression of N^{intra.GS.Scer\UAS} under the control of Scer\GAL4^tub has no effect on the maintenance of mam⁸ follicle stem cell clones.

Expression of Su(H)^{Scer\UAS.cSa.T:Hsim\VP16} under the control of Scer\GAL4^tub has no effect on the maintenance of mam⁸ follicle stem cell clones.

(Vied and Kalderon, 2009)

No significant effect on the sca^MSKF mutant phenotype.

(Hu et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mam^S2-29