FlyBase Allele Report: Dmel\Orc2[2]

General Information

Symbol

Dmel\Orc2²

Species

D. melanogaster

Name

FlyBase ID

FBal0010550

Feature type

allele

Associated gene

Dmel\Orc2

Associated Insertion(s)

Carried in Construct

Also Known As

ORC2^fs293, fs293

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Landis et al., 1997, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Cytologically normal.

(Landis et al., 1997)

Description

Nucleotide substitution: A961C Nucleotide substitution: G1003A. Amino acid replacement: T321P. Amino acid replacement: E335K.

(Landis et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:13,966,036..13,966,036 [+]

Nucleotide change:

A13966036C

Reported nucleotide change:

A961C

Amino acid change:

T321P | Orc2-PA

Reported amino acid change:

T321P

Comment:

One of two nucleotide changes in mutant. Less likely of the two changes to cause phenotype.

(Landis et al., 1997)

point_mutation

3R:13,966,078..13,966,078 [+]

Nucleotide change:

G13966078A

Reported nucleotide change:

G1003A

Amino acid change:

E335K | Orc2-PA

Reported amino acid change:

E335K

Comment:

One of two nucleotide changes in mutant. Probable cause of phenotype.

(Landis et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Tearle and Nusslein-Volhard, 1987)

female sterile | recessive

(Landis et al., 1997, Pak et al., 1997)

suppressor of variegation, with In(1)w^m4h

(Pak et al., 1997)

viable

(Tearle and Nusslein-Volhard, 1987)

Phenotype Manifest In

egg chorion

(Landis et al., 1997, Tearle and Nusslein-Volhard, 1987)

Detailed Description

Statement

Reference

Chorion gene amplification is depressed in Orc2² egg chambers.

(Asano and Wharton, 1999)

Mutant eggs have defective chorions.

(Landis et al., 1997)

Orc2² females produce thin or no chorion.

(Tearle and Nusslein-Volhard, 1987)

eggs of homozygous females defective in late-deposited outer layers of endochorion; endochorionic material deficient. Late-gene expression as measured by mRNA or protein levels defective; deficiency in the level of chorion-gene amplification in follicle cells. Homozygous females weakly fertile; produce eggs with grossly defective chorions; thin, missing most appendages and permeable. All chorion proteins reduced in abundance; chorion gene amplification reduced to 10% wild-type level (Snyder et al., 1986). Heterozygotes with lethal alleles more extreme and sterile.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Orc2^γ4/Orc2² is rescued by Orc2^GFP.N

(Baldinger and Gossen, 2009)

Orc2^γ4/Orc2² is rescued by Orc2^GFP.C

(Baldinger and Gossen, 2009)

Orc2² is rescued by Orc2^+t7.5

(Landis et al., 1997)

Comments

The chorion phenotype is rescued by Orc2^+t7.5.

(Landis et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Nusslein-Volhard.

Comments

Ovaries derived from homozygous females show greatly reduced incorporation of BrdU compared to wild-type, and labeling is mosaic in intensity among the follicle cells.

(Calvi et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

88Ab²

(Tearle and Nusslein-Volhard, 1987)

ORC2^fs293

(Zhang and Tower, 2004, Asano and Wharton, 1999)

Orc2²

fs(2)293

(Whittaker et al., 2000)

fs(3)293

(Calvi et al., 1998)

fs(3)293-19

fs293

(Landis et al., 1997, Pak et al., 1997)

k43²⁹³

(Tower, 2004)

k43^fs293

(Pflumm and Botchan, 2001)

l(3)88Ab²

orc2^fs293

(Kim and Orr-Weaver, 2011)

Name Synonyms

Secondary FlyBase IDs

References (13)

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