FlyBase Allele Report: Dmel\Spc25[A34-1]

General Information

Symbol

Dmel\Spc25^A34-1

Species

D. melanogaster

Name

FlyBase ID

FBal0010460

Feature type

allele

Associated gene

Dmel\Spc25

Associated Insertion(s)

Carried in Construct

Also Known As

l(3)A34-1

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Schittenhelm et al., 2007)

Mutagen

ethyl methanesulfonate

(Hilliker et al., 1981)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?130term.

(Williams et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:12,962,760..12,962,760 [+]

Nucleotide change:

G12962760T

Amino acid change:

E130term | Spc25-PA

Reported amino acid change:

?130term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change as a nonsense mutation at the beginning of exon 3 that truncates the gene product to 129 residues.

(Williams et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsocentral bristle

(Hilliker et al., 1980)

larval neuroblast (with Df(3R)ry75)

(Williams et al., 2007)

larval neuroblast (with Spc25²²)

(Williams et al., 2007)

larval neuroblast (with Spc25^mitch-1)

(Williams et al., 2007)

macrochaeta

spermatocyte (with Spc25^mitch-1)

(Williams et al., 2007)

Detailed Description

Statement

Reference

Brain neuroblasts in Spc25^A34-1/Spc25^19B third instar larvae show high levels of aneuploidy. Many anaphase cells have lagging chromosomes.

21.5% of mitotic cells in Spc25^mitch-1/Spc25^A34-1 larval brains have overcondensed chromosomes, and the mitotic index is reduced compared to wild type. The ratio of mitotic cells in prometaphase/metaphase to those in anaphase/telophase is increased compared to wild type.

61.9% of mitotic cells in Spc25^A34-1/Df(3R)ry75 larval brains have overcondensed chromosomes. The ratio of mitotic cells in prometaphase/metaphase to those in anaphase/telophase is increased compared to wild type.

Instead of arresting in mitosis, Spc25^mitch-1/Spc25^A34-1 and Spc25^A34-1/Df(3R)ry75 larval neuroblasts treated with colchicine prematurely enter anaphase and show a high incidence of precocious sister chromatid separation.

Spc25^mitch-1/Spc25^A34-1 spermatocytes show defects during the two meiotic divisions. Bivalent congression during meiosis I and II is disrupted and chromosome segregation during anaphase and telophase of both meiotic divisions is defective.

(Williams et al., 2007)

Semilethal.

(Hilliker et al., 1981)

Semilethal when heterozygous with l(3)87Da¹. Adult survivors exhibit variation in dorsocentral bristle number and length and females greatly outnumber males.

(Hilliker et al., 1980)

Surviving heterozygotes with l(3)87Da¹ exhibit variation in dorsal central bristle number and length and females greatly outnumber males. l(3)87Da²/ l(3)87Da³ survivors have normal morphology.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement