FB2024_03 , released June 25, 2024
Allele: Dmel\Lim36
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General Information
Symbol
Dmel\Lim36
Species
D. melanogaster
Name
FlyBase ID
FBal0008653
Feature type
allele
Associated gene
Dmel\Lim3
Associated Insertion(s)
Carried in Construct
Also Known As
Lim3BD6, Bd6
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

9bp deletion (bases 1,698-1706) in the 3' untranslated region which may affect the stability of the mRNA.

(Thor et al., 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:19,080,112..19,080,120 [-]
Comment:

A 9bp deletion in the 3 UTR which may affect mRNA stability.

(Thor et al., 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In Lim36/+ embryos less than 1% of muscles 12 and 13 fail to be innervated and less than 3% of ISNb axons leave the ventral muscle field and target the transverse nerve (TN) fascicle. In stage 16 Lim36/Df(2L)TW130 embryos, 19% of hemisegments have no transverse nerve, while 38% of hemisegments have transverse nerve fasciculation defects.

      (Certel and Thor, 2004)

      Most hemizygotes do not pupariate, remaining as second or third instar larvae up to 8 or 9 days after egg laying. A few hemizygotes do pupariate 8 to 11 days after egg laying, and these pupae generally develop into pharate adults that do not eclose. These pharate adults show a complete lack of differentiated cuticle on the abdomen, and have reduced cuticle formation on the thorax and head.

      (Wright, 1996)

      Hemizygous Lim36/Df(2L)TW130 animals do not survive to the third larval instar stage.

      (McCrady and Tolin, 1994)

      strong allele in which second and third instar larvae persist until days eight and nine; few survive to pharate-adult stage with incomplete sclerotization.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Lim36, vvl[+]/vvldfr-B129 has abnormal neuroanatomy | dominant phenotype, enhanceable by tupisl-1/tup[+]

      (Certel and Thor, 2004)

      Lim3[+]/Lim36, vvldfr-B129 has abnormal neuroanatomy | dominant phenotype, enhanceable by tupisl-1/tup[+]

      (Certel and Thor, 2004)

      Lim36 has abnormal neuroanatomy | dominant phenotype, enhanceable by Df(2L)TE35D-19/+

      (Certel and Thor, 2004)

      Lim36 has abnormal neuroanatomy | dominant phenotype, enhanceable by vvl[+]/vvldfr-B129

      (Certel and Thor, 2004)
      Enhancer of
      Statement
      Reference

      Lim3[+]/Lim36 is an enhancer of abnormal neuroanatomy | dominant phenotype of vvldfr-B129

      (Certel and Thor, 2004)

      Lim3[+]/Lim36 is an enhancer of abnormal neuroanatomy | dominant phenotype of tupisl-1, vvl[+]/vvldfr-B129

      (Certel and Thor, 2004)

      Lim3[+]/Lim36 is an enhancer of abnormal neuroanatomy | dominant phenotype of tupisl-1/tup[+], vvldfr-B129

      (Certel and Thor, 2004)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In Lim36/+ embryos less than 1% of muscles 12 and 13 fail to be innervated and less than 3% of ISNb axons leave the ventral muscle field and target the transverse nerve (TN) fascicle. The expressivity of these phenotypes is enhanced to 29% and 34% respectively by vvldfr-B129/+. In tupisl-1, Lim36/+; vvldfr-B129/+ embryos, the ISNb motoneurons fail to project to and innervate their specific target muscles, including muscles 12 and 13. In stage 16 Lim36/+ Df(2L)TE35D-19/+ embryos, 17% of hemisegments have no transverse nerve, while 25.5% of hemisegments have transverse nerve fasciculation defects. In stage 16 tup1/+ Lim36/+ Df(2L)TE35D-19/+ embryos, 9.3% of hemisegments have no transverse nerve, while 36% of hemisegments have transverse nerve fasciculation defects. In stage 16 tup1/+ Lim36/+ Df(2L)TE35D-19/+ embryos, 4% of hemisegments have no transverse nerve, while 46% of hemisegments have transverse nerve fasciculation defects.

      (Certel and Thor, 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Wright.

      Comments
      Comments

      Strong allele.

      (Thor et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      Bd6
      (Thor et al., 1999, Wright, 1996)
      Lim36
      Lim3BD6
      (Certel and Thor, 2004)
      l(2)37Bd6
      (Stathakis et al., 1995)
      l(2)621
      lim3Bd6
      (Santiago and Bashaw, 2017, Thor et al., 1999)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)