FlyBase Allele Report: Dmel\heix[k11403]

General Information

Symbol

Dmel\heix^k11403

Species

D. melanogaster

Name

FlyBase ID

FBal0008538

Feature type

allele

Associated gene

Dmel\heix

Associated Insertion(s)

P{lacW}heix^k11403

Carried in Construct

Also Known As

k11403

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Roote, 1995.5.4)

Progenitor genotype

Associated Insertion(s)

P{lacW}heix^k11403

Cytology

Description

Inserted in the 5' UTR of the heix transcript.

(Xia et al., 2015)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,299,591..16,299,591 [+]

(Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Spradling et al., 1999, Roote, 1995.5.4, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of lymphoma

CEA with heix¹

(Dragh et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Pink1^B9

(Vos et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2L)RA5)

(Xia et al., 2015)

lethal (with heix¹)

(Xia et al., 2015)

lethal | recessive

(Ashburner et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

melanotic mass phenotype (with Df(2L)RA5)

(Xia et al., 2015)

melanotic mass phenotype (with heix¹)

(Xia et al., 2015)

Phenotype Manifest In

embryonic/larval hemocyte | increased number (with Df(2L)RA5)

(Xia et al., 2015)

embryonic/larval hemocyte | increased number (with heix¹)

(Xia et al., 2015)

lamellocyte (with Df(2L)RA5)

(Xia et al., 2015)

lamellocyte (with heix¹)

(Xia et al., 2015)

melanotic mass (with Df(2L)RA5)

(Xia et al., 2015)

melanotic mass (with heix¹)

(Xia et al., 2015)

mitochondrion (with Df(2L)RA5)

(Xia et al., 2015)

primary lobe of embryonic/larval lymph gland (with Df(2L)RA5)

(Xia et al., 2015)

primary lobe of embryonic/larval lymph gland (with heix¹)

(Xia et al., 2015)

secondary lobe of embryonic/larval lymph gland (with Df(2L)RA5)

(Xia et al., 2015)

secondary lobe of embryonic/larval lymph gland (with heix¹)

(Xia et al., 2015)

Detailed Description

Statement

Reference

Most heix^k11403/Df(2L)RA5 and heix^k11403/heix¹ flies produce melanotic masses in larval or pupal stage. Mutant larvae present an enormous overgrowth of the lymph gland. This event is coupled with a dramatic increase of circulating hemocytes and an aberrant differentiation of lamellocytes. The size of primary lobes in heix^k11403/Df(2L)RA5 and heix^k11403/heix¹ mutant lymph glands is about two times and four times larger than that in the control, respectively; the size of secondary lobes in heix^k11403/Df(2L)RA5 flies is about five times larger, and in heix^k11403/heix¹ third instar larvae, they are approximately twenty times larger than that observed in controls.

Primary and secondary lobes in heix^k11403/Df(2L)RA5 lymph glands lose their architectural structure.

The lymph glands and fat body of heix^k11403/Df(2L)RA5 third instar larvae show a mitochondria morphological defect.

(Xia et al., 2015)

Feeding hemizygous animals with the MK-4 form of vitamin K[[2]], but not with menadione (a synthetic precursor that is prenylated to form vitamin K[[2]]) or with ubiquinone significantly improves their survival rate.

(Vos et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

heix^k11403/heix[+] is an enhancer of abnormal flight phenotype of Pink1^B9

(Vos et al., 2012)

heix^k11403/heix[+] is an enhancer of visible | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

NOT Enhancer of

Statement

Reference

heix^k11403/heix[+] is a non-enhancer of lethal | embryonic stage | recessive | partially phenotype of peb³⁰⁸

(Wilk et al., 2004)

Suppressor of

Statement

Reference

heix^k11403/heix[+] is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

heix^k11403/heix[+] is a non-suppressor of lethal | recessive | partially | embryonic stage phenotype of peb³⁰⁸

(Wilk et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

heix^k11403/heix[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

heix^k11403/heix[+] is an enhancer of wing | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Suppressor of

Statement

Reference

heix^k11403/heix[+] is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

heix^k11403/Df(2L)RA5 is partially rescued by heix^UAS.cVa/Scer\GAL4^αTub84B.PL

(Xia et al., 2015)

Comments

Phenotypes of lethality, lymph gland overgrowth, circulating hemocytes increase and aberrant differentiation that appear in heix^k11403/Df(2L)RA5 flies are efficiently rescued by heix^Scer\UAS.cVa expression under the control of Scer\GAL4^αTub84B.PL - the majority of these flies survive and are fertile.

(Xia et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11031

y¹ w^67c23; P{lacW}heix^k11403/CyO

Kyoto

111306

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}heix^k11403 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(Roote, 1995.5.4)

Comments

Complements: dac^P.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

heix^k11403

(Dragh et al., 2017, Xia et al., 2015)

k11403

(Vos et al., 2012, Ashburner et al., 1999)

l(2)35Fc^114/3

l(2)35Fc^k11403

(Johannes and Preiss, 2002, Ashburner et al., 1999.10.12, Roote, 1995.5.4, BDGP Project Members, 1994-1999)

l(2)k11403

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close