FlyBase Allele Report: Dmel\esc[21]

General Information

Symbol

Dmel\esc²¹

Species

D. melanogaster

Name

FlyBase ID

FBal0003841

Feature type

allele

Associated gene

Dmel\esc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Gindhart and Kaufman, 1995)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: S301F. S301F falls in the WD5 domain.

(Tie et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:11,827,836..11,827,836 [-]

Nucleotide change:

C11827836T

Amino acid change:

S301F | esc-PA

Reported amino acid change:

S301F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Tie et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Parrish et al., 2007)

abnormal neuroanatomy | somatic clone

(Parrish et al., 2007)

Phenotype Manifest In

dendrite & dorsal multidendritic neuron ddaC | somatic clone

(Parrish et al., 2007)

dendrite & dorsal multidendritic neuron ddaC | third instar larval stage

(Parrish et al., 2007)

Detailed Description

Statement

Reference

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for esc²¹ show a range of dendritic defects in the third instar larva. Homozygous class IV ddaC clones show a significant reduction in dendrite branching and total dendritic length compared to controls.

Homozygotes can survive through larval stages and often into adulthood.

Dendrites of class IV dendrite arborisation (da) neurons are indistinguishable from wild-type controls in homozygotes throughout embryogenesis and early larval stages. However, beginning at 48 hours after egg laying (AEL), defects in class IV dendrites become evident in homozygotes. By 96 hours AEL there is a significant reduction both in the number of terminal dendritic branches and in total dendritic length in the homozygotes. In some severe cases (approximately 10% of neurons sampled), there is a complete loss over major regions of the dendritic field. Analysis of dendrite development between 72 and 96 hours AEL shows that homozygous class IV dendrites show extensive growth during this period, as occurs in controls, but in the mutants, some major dendritic trunks show signs of severing (which is never seen in controls). By 96 hours AEL, the severed dendrites are almost completely eliminated in the mutants. Mutant dendrites show a greater number and apparent rate of retraction events than controls.

Class IV da neurons show no sign of cell death in mutant third instar larvae and they persist through metamorphosis and into adulthood.

Class IV da neurons enter the central nervous system at the appropriate position in mutant larvae and the overall morphology of the neurons in the central nerve cord is indistinguishable from that of controls.

Heterozygous males do not have ectopic sex combs on the second or third legs.

(Parrish et al., 2007)

Heterozygous males do not have ectopic sex combs on the second and third legs.

(Qi et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

esc²¹/esc[+] is an enhancer of visible | dominant phenotype of Pc³

(Parrish et al., 2007)

NOT Enhancer of

Statement

Reference

esc²¹/esc[+] is a non-enhancer of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-enhancer of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of Pc¹⁵

(Parrish et al., 2007)

esc²¹/esc[+] is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of E(z)⁶³

(Parrish et al., 2007)

Suppressor of

Statement

Reference

esc²¹/esc[+] is a suppressor of visible phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

esc²¹/esc[+] is a suppressor of visible phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Ji et al., 2012)

esc²¹/esc[+] is a suppressor of visible | dominant | homeotic phenotype of ash1¹⁷, trx^B11/trx[+]

(Gildea et al., 2000)

esc²¹/esc[+] is a suppressor of visible | dominant | homeotic phenotype of brm[+]/brm², trx^E2

(Gildea et al., 2000)

NOT Suppressor of

Statement

Reference

esc²¹/esc[+] is a non-suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of E(z)⁶³

(Parrish et al., 2007)

esc²¹/esc[+] is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of Pc¹⁵

(Parrish et al., 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

esc²¹/esc[+] is an enhancer of sex comb | ectopic phenotype of Pc³

(Parrish et al., 2007)

esc²¹/esc[+] is an enhancer of mesothoracic leg phenotype of Pc³

(Parrish et al., 2007)

esc²¹/esc[+] is an enhancer of metathoracic leg phenotype of Pc³

(Parrish et al., 2007)

NOT Enhancer of

Statement

Reference

esc²¹/esc[+] is a non-enhancer of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-enhancer of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-enhancer of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of Pc¹⁵

(Parrish et al., 2007)

esc²¹/esc[+] is a non-enhancer of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of E(z)⁶³

(Parrish et al., 2007)

Suppressor of

Statement

Reference

esc²¹/esc[+] is a suppressor of eye phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

esc²¹/esc[+] is a suppressor of 1st posterior cell phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Ji et al., 2012)

esc²¹/esc[+] is a suppressor of adult metathoracic segment phenotype of ash1¹⁷, trx^B11/trx[+]

(Gildea et al., 2000)

esc²¹/esc[+] is a suppressor of adult metathoracic segment phenotype of brm[+]/brm², trx^E2

(Gildea et al., 2000)

NOT Suppressor of

Statement

Reference

esc²¹/esc[+] is a non-suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

esc²¹/esc[+] is a non-suppressor of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of E(z)⁶³

(Parrish et al., 2007)

esc²¹/esc[+] is a non-suppressor of dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype of Pc¹⁵

(Parrish et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is not affected by combination with a single copy of esc²¹.

(Morimoto et al., 2016)

The addition of esc²¹/+ does not alter the dendritic defects seen in single cell class IV dendrite arborisation (da) neuron clones that are homozygous for Pc¹⁵ or homozygous for E(z)⁶³.

The ectopic sex comb phenotype seen on the second and third legs of Pc³/+ males is enhanced by esc²¹/+.

(Parrish et al., 2007)

rept⁰⁶⁹⁴⁵ shows little or no interaction (in terms of ectopic sex comb phenotype) when in double heterozygous combination with esc²¹.

(Qi et al., 2006)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is not affected by combination with a single copy of esc²¹.

(Morimoto et al., 2016)

Complementation and Rescue Data

Rescued by

esc²¹/esc^M20 is rescued by Scer\GAL4^arm.PS/esc^UAS.Tag:FLAG

(Langlais et al., 2012)

esc²¹ is rescued by Scer\GAL4^elav-C155/esc^UAS.cUa

(Parrish et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

107136

esc²¹ b¹ cn¹/In(2LR)Gla, wg^Gla-1; ca¹ awd^K

Notes on Origin

Discoverer

Struhl.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

esc²¹

(Bahrampour et al., 2019, Curt et al., 2019, Dardalhon-Cuménal et al., 2018, Yaghmaeian Salmani et al., 2018, Ji et al., 2012, Langlais et al., 2012, Parrish et al., 2009, Parrish et al., 2007, Christensen and Cook, 2006.12.5, Qi et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (16)

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