Date: Tue, 05 Dec 2006  14:55:26  -0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC237
Cc: Jill Gresens <jgresensXXXX>, Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufmanXXXX
Isolation and characterization of Df(2L)BSC237
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC237 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}e03051 and P{XP}escl[d01514]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{RB}e03051/P{XP}escl[d01514] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.RB3}BSC237 from the segment 
of PBac{RB}e03051 to the left of its FRT site and the segment of 
P{XP}escl[d01514] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al 
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' 
for the RB3' minus primer in the Hybrid PCR protocol in the 
Supplementary Methods. The cytological breakpoints of Df(2L)BSC237 
predicted from the Release 4 genomic coordinates of the transposable 
element insertions sites are 32F2;33B5. It failed to complement 
crol[04418] and esc[21].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX