adult thorax & adult epidermis
Homozygous clones in the adult thorax result in a vortex phenotype.
Homozygous clones in the wing result in deformed wings with blisters.
When mutant clones are induced in the wing, a wing blister appears within a few minutes of eclosion of the adult flies from the pupal case.
Clones of mutant cells in the pupal wing still contain the transalar microtubule arrays that are normally seen spanning the wing cells at this stage.
At the ultrastructural level, homozygous embryos show a separation of the apical extracellular matrix (cuticle) from the apical surface of the epidermis. All elements of the external epicuticle appear to be properly formed and remain in tight contact with the upper layers of the endocuticle. However, the chitin layer of the endocuticle has disintegrated, especially towards its basal side. An electron dense layer, presumably corresponding to a defective deposition zone, is faint and discontinuous compared to the wild-type deposition zone, and its typical tight contact to the chitin layer above is lost. On the apical surface of the mutant epidermal cells, the typical microvilli can still be seen, often attached to the remaining fragments of the deposition zone. Junctional structures within the mutant epidermal cells appear normal. The apical and basal junctions of tendon cells are intracellularly connected by large microtubule bundles in the mutant embryos and at the apical side, long tonofilaments extend away into the extracellular space (as occurs in wild type). Normally, tonofilaments anchor in the cuticle, however, in the mutant embryos, they appear to have been pulled out. The basal adhesive junctions of the tendon cells appear normal in the mutant embryos and the attachment of the basal surface of the rest of the epidermal cells to the basement membrane also appears normal.
Transheterozygotes with dpD are lethal.
dpylv1/dp[+] is an enhancer of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU
dpy[+]/dpylv1 is an enhancer of wing blade posterior compartment | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU
dpylv1 is an enhancer of wing phenotype of EogtNIG.9867R, Scer\GAL4en-e16E
dplv1 dominantly enhances the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa, such that the phenotype is seen at 22.5[o]C.
A dplv1 heterozygous background markedly increases the penetrance of wing blisters caused by expression of EogtNIG.9867R under the control of Scer\GAL4en-e16E.
'Failure to complement' statements based on recessive lethality.
Bridges, 3 March 1920.
