FlyBase Allele Report: Dmel\aurA[3]

General Information

Symbol

Dmel\aurA³

Species

D. melanogaster

Name

FlyBase ID

FBal0000801

Feature type

allele

Associated gene

Dmel\aurA

Associated Insertion(s)

Carried in Construct

Also Known As

aurA²⁸⁷, aur^287-19

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: H3Y. Amino acid replacement: D47A. Amino acid replacement: T90P. Amino acid replacement: R162C.

(Giet et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:11,963,298..11,963,298 [+]

Nucleotide change:

C11963298T

Amino acid change:

H3Y | aurA-PA

Reported amino acid change:

H3Y

Comment:

One of three amino acid changes observed in mutant (not including observed polymorphism); probably not primarily responsible for reduced kinase activity. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Giet et al., 2002)

point_mutation

3R:11,963,623..11,963,623 [+]

Nucleotide change:

A11963623C

Amino acid change:

T90P | aurA-PA

Reported amino acid change:

T90P

Comment:

(Giet et al., 2002)

point_mutation

3R:11,963,901..11,963,901 [+]

Nucleotide change:

C11963901T

Amino acid change:

R162W | aurA-PA

Reported amino acid change:

R162C

Comment:

Arg codon is CGG in reference strain and CGC in reported sequence (FBrf0080055). A CGC to TGC change is presumed to account for the reported R162C change in aurA³ (FBrf0144945). The same nucleotide change would cause a R162W mutation in the reference strain. The reduced kinase activity of the mutant is thought to be due to the mutation of this conserved Arg in the ATP binding pocket in subdomain I of the catalytic domain. Two other nucleotide changes were reported in the aurA³ mutant (not including an observed polymorphism). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Giet et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with Df(3R)T-61)

(Glover et al., 1995)

abnormal mitotic cell cycle | maternal effect

(Giet et al., 2002)

female sterile (with aurA^87Ac-3)

(Glover et al., 1995)

female sterile (with aurA^87Ac-4)

(Glover et al., 1995)

female sterile (with aurA^87Ac-5)

(Glover et al., 1995)

female sterile | recessive

(Glover et al., 1995)

sterile

(Inoue and Glover, 1998)

Phenotype Manifest In

microtubule | maternal effect

(Giet et al., 2002)

primordial germ cell (with aurA^87Ac-3)

(Lerit and Gavis, 2011)

Detailed Description

Statement

Reference

aur³/aur^87Ac-3 embryos have a reduced number of pole cells compared to controls and the pole cells that do form often have greatly reduced germ plasm.

(Lerit and Gavis, 2011)

In embryos from aur³ mutant mothers, the astral microtubules at the spindle poles during mitoses are short and reduced in density compared to wild-type.

(Giet et al., 2002)

rl^Su14 and Dsor1^Su1 have no effect on the mutant phenotype.

(Inoue and Glover, 1998)

Larval brain neuroblasts have wild type mitotic figures in homozygotes. aur³/Df(3R)T-61 larval brain neuroblasts show a low but significant number of abnormal, polyploid, mitotic figures.

(Glover et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

aurA^87Ac-6

(Glover et al., 1995)

aurA^87Ac-5

(Glover et al., 1995)

aurA^87Ac-4

(Glover et al., 1995)

aurA^87Ac-3

(Glover et al., 1995)

aurA^87Ac-2

(Glover et al., 1995)

aurA^87Ac-1

(Glover et al., 1995)

Rescued by

aurA³ is rescued by aurA^+t16.2

(Glover et al., 1995)

aurA³ is rescued by aurA^+t3.7

(Glover et al., 1995)

aurA³ is rescued by aurA^+t9

(Glover et al., 1995)

Not rescued by

aurA³ is not rescued by aurA^VI

(Glover et al., 1995)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Kinase activity of gene product is reduced.

(Giet et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

aur^287-19

(Inoue and Glover, 1998, Glover et al., 1995)

aur²⁸⁷

(Tearle and Nusslein-Volhard, 1987)

aur³

aurA²⁸⁷

(Moon and Matsuzaki, 2013, Zhang et al., 2009, Giet et al., 2002)

aurA³

(Lerit and Gavis, 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

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