FB2024_03 , released June 25, 2024
Allele: Dmel\aurA87Ac-5
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General Information
Symbol
Dmel\aurA87Ac-5
Species
D. melanogaster
Name
FlyBase ID
FBal0000806
Feature type
allele
Associated gene
Dmel\aurA
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

neomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Glover et al., 1995)

neomorphic allele - genetic evidence

(Giet et al., 2002)
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

Double mutant. Amino acid replacement: D47A. Amino acid replacement: E212K. E212 is normally absolutely conserved.

(Glover et al., 1995)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:11,964,021..11,964,021 [+]
Nucleotide change:

G11964021A

Amino acid change:

E202K | aurA-PA

Reported amino acid change:

E212K

Comment:

An additional 10aa in the reported aurA CDS (FBrf0080055) relative to the reference CDS accounts for the difference in the reported and reference amino acid locations of the change in the mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Glover et al., 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      aster & neuroblast & larva (with Df(3R)T-61)

      (Giet et al., 2002)

      centriole & neuroblast & larva | ectopic (with Df(3R)T-61)

      (Giet et al., 2002)

      centrosome & neuroblast & larva

      (Wang et al., 2006)

      centrosome & neuroblast & larva (with Df(3R)T-61)

      (Giet et al., 2002)

      embryonic/larval brain

      (Wang et al., 2006)

      microtubule & neuroblast & larva (with Df(3R)T-61)

      (Giet et al., 2002)

      mitotic metaphase & larval brain

      (Giet et al., 2002)

      mitotic metaphase & larval brain (with Df(3R)P-79)

      (Giet et al., 2002)

      mitotic metaphase & larval brain (with Df(3R)T-47)

      (Giet et al., 2002)

      mitotic metaphase & larval brain (with Df(3R)T-61)

      (Giet et al., 2002)
      Detailed Description
      Statement
      Reference

      Centrosome number is abnormal in mutant larval neuroblasts, with one to three centrosomes being seen.

      (Wang et al., 2006)

      The larval brains of aur87Ac-5 homozygotes, as well as those of aur87Ac-5/Df(3R)T-47, aur87Ac-5/Df(3R)T-61 and aur87Ac-5/Df(3R)P-79 animals, have a significantly elevated mitotic index with the majority of cells delayed in a metaphase-like state. A substantial proportion of metaphase chromosomes seen in these cells are arranged in circular arrays (indicating monopolar spindles). A small proportion of cells in these brains are aneuploid or polyploid. Note, the hemizygotes have less cells with circular arrays than homozygotes, suggesting that aur87Ac-5 might be neomorphic. In dividing aur87Ac-5/Df(3R)T-61 larval brain neuroblasts, astral microtubules are short and reduced in number throughout mitosis compared to wild-type. Many of the spindles in these cells are bipolar but mono-astral, with the remaining diminutive aster nucleated around a fragmented centrosome, frequently only present at one pole. Poles of bipolar spindles in such mutant brains either showed focused microtubules with no evidence of an organized centrosome, or spindle microtubules nucleated from bodies containing multiple centrioles.

      (Giet et al., 2002)

      aur87Ac-5/aur87Ac-3 larval brain neuroblasts show very few normal metaphase and anaphase figures, and many circular mitotic figures. Larval brain neuroblasts from aur87Ac-4/aur87Ac-5 show multiple mitotic figures with only one centrosome in the circular mitotic figures. The single centrosome is larger than those of bipolar spindles, consistent with them being an aggregate of duplicated centrosomes.

      (Glover et al., 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially complements

      aurA3

      (Glover et al., 1995)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      aur87Ac-5
      (Wang et al., 2006)
      aure209
      (Dobbelaere et al., 2008)
      aurA87Ac-5
      aurAe209
      (Giet et al., 2002)
      aure209
      (Giet et al., 2002)
      ck10e209
      (Glover et al., 1995)
      e209
      (Gausz et al., 1981)
      l(3)87Ac5
      Name Synonyms
      Secondary FlyBase IDs
        References (5)