brm² ash1⁶ double heterozygotes show partial transformation of the 5th abdominal segment to fourth abdominal segment, but do not hold out their wings at any significantly higher frequency than that seen in brm²/+ single heterozygotes.

Heterozygotes of ash1¹, ash1⁶, and ash1²⁸ with brm² have homeotic transformations of the third to the second leg. The expressivity of the transformed leg is variable, some have only a second leg apical bristle while others have a second leg preapical bristle as well. There is a lower penetrance of third leg transformations among flies heterozygous for ash1¹, ash1⁶, and ash1²⁸ with brm⁵. Degree of penetrance corresponds to the severity of the ash1 allele (ash1¹ < ash1⁶ < ash1²⁸). There is a lower penetrance of partial homeotic transformation of the haltere to the wing, or dorsal metathoracic cuticle to mesothoracic cuticle, and an even lower penetrance of first leg to second leg transformations.

Flies heterozygous for both Df(3R)red-P93 and ash1⁶ express partial transformation of halteres to wings or third legs to second legs, there is also evidence of a maternal as well as zygotic component to the interaction. Flies heterozygous for both Df(3R)P9 (deletes entire BX-C) and ash1⁶ express transformations from metathorax to mesothorax, this interaction depends on an ash-1 maternal effect. Suppresses the dominant extra sex combs phenotype caused by a heterozygosity for Df(3L)Pc.