FlyBase Allele Report: Dmel\βTub85D[7]

General Information

Symbol

Dmel\βTub85D⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0000028

Feature type

allele

Associated gene

Dmel\βTub85D

Associated Insertion(s)

Carried in Construct

Also Known As

B2t⁷

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Fuller et al., 1988)

loss of function allele

(Hutchens et al., 1997)

Mutagen

ethyl methanesulfonate

(Fackenthal et al., 1995, Fuller et al., 1988)

Progenitor genotype

Cytology

Description

Amino acid replacement: D114N. Nucleotide substitution: GAT to AAT.

(Fackenthal et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,409,713..9,409,713 [-]

Nucleotide change:

G9409713A

Reported nucleotide change:

G?A

Amino acid change:

D114N | betaTub85D-PA

Reported amino acid change:

D114N

(Fackenthal et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | male | recessive

(Fuller et al., 1988, Fuller, 1986)

abnormal meiotic cell cycle | male (with βTub85Dⁿ)

(Fuller et al., 1988)

electrophoretic variant

(Fuller et al., 1988)

male sterile (with βTub85Dⁿ)

(Fuller et al., 1988)

male sterile | recessive

Phenotype Manifest In

axoneme

(Fuller et al., 1988)

meiotic cell cycle | male

(Fuller, 1986)

microtubule

(Green et al., 1990)

Nebenkern derivative

(Fuller et al., 1988)

spermatid axoneme

(Fuller, 1986)

testis

(Green et al., 1990)

Detailed Description

Statement

Reference

Affects microtubule function during spermatogenesis.

(Green et al., 1990)

Chromosome movement during meiosis usually appears normal in homozygous males, but failure of cytokinesis after meiosis I or II is frequent, with two or four nuclei found associated with a single, abnormally large mitochondrial derivative in the developing spermatids. Smaller mitochondrial derivatives than normal are also seen. Nuclear shaping during spermatogenesis occurs normally, but nuclear alignment is defective. Spermatid cysts often contain fewer axonemes than normal, and the axonemes generally lack one or both central pair microtubules. The axonemes are shorter than normal. Broken axonemes or axoneme fragments are common. Some spermatid cyst elongation takes place. Chromosome segregation during meiosis is sometimes abnormal in βTub85D⁷/βTub85Dⁿ males. βTub85D⁷/βTub85Dⁿ males are sterile.

(Fuller et al., 1988)

Although relatively well organised near the basal body, the axonemes in the spermatids of homozygous males are abnormally short, appear to fray apart as the distance from the basal body exceeds more than a few μm and lack one or both central pair microtubules. Occasional failure of cytokinesis after meiosis I or II is seen. Nuclear shaping is normal.

(Fuller, 1986)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

βTub85D⁷ is a suppressor of male sterile phenotype of βTub60D::βTub85D^star

(Fackenthal et al., 1995)

Other

Statement

Reference

βTub85D⁷, wrl¹ has male fertile phenotype

(Green et al., 1990)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

βTub85D⁷ heterozygotes partially rescue the male sterile phenotype of βTub60D::βTub85D^star, some motile sperm is present in the seminal vesicles.

(Fackenthal et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Basic electrophoretic variant. The protein encoded by βTub85D⁷ is stable and capable of forming an αβ tubulin heterodimer.

(Fuller et al., 1988)

Class II allele.

(Fackenthal et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

B2t⁷

(Hutchens et al., 1997, Fackenthal et al., 1995, Fuller, 1993, Hoyle and Raff, 1990, Fuller et al., 1988)

Tβ2⁷

β2t⁷

(Fuller, 1986)

βTub85D⁷

Name Synonyms

Secondary FlyBase IDs

References (7)

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