Gene conversion using P{ΔmiRNA.donor} as the donor. The gene conversion event has resulted in most of the genomic sequence that encodes both the mir-iab-4 and mir-iab-8 microRNAs (these microRNAs are encoded on opposite strands) being replaced with sequence encoding the HindIII and RS(I-SceI) enzyme sites.
Female fertile and male sterile in transheterozygous combination with T(2;3)iab445.
Male and female fertile in transheterozygous combination with In(3LR)iab333, Ab(?;3)iab3B1, In(3R)iab9Uab1, Tp(3;Y)Abd-BS10, T(2;3)iab965, Ab(?;3)iab386A or T(2;3;4)iab4302.
Male and female sterile in transheterozygous combination with T(2;3)iab6105, In(3R)iab6G, In(3R)iab7SGA, In(3R)iab7Spth, Tp(3;2)iab5843 or Ab(?;3)iab-4186.
Weakly fertile transheterozygous combination with Ab(?;3)iab42330.
Homozygotes show partial lethality. Some lethality is seen during the embryonic stage and some during the pupal stage.
Homozygous males show a significant decrease in median lifespan compared to controls.
Homozygous males and females are sterile.
Homozygous adults show no evidence of segmental transformation in the abdominal cuticle of either sex. Homozygous adults of both sexes are sterile. Females have ovaries with eggs of normal size, but only very rare individuals ever lay an egg, even after mating with wild-type males, and these eggs never hatch. Males have morphologically normal testes containing motile sperm. Homozygous females mate with wild-type males as readily as do heterozygous sibling females. Homozygous males show normal courtship except that they never complete copulation. They mount the females, but do not bend their abdomens quite far enough to mate.
