97D2;97D9
97D2;97D9
bk1 << l(3)97Df << l(3)97De << bk2 << Ets97D
Df(3R)Tl-X/Df(3R)ro-XB3 embryos show altered targeting of the ISNb pathway. The nerve endings at the cleft between muscles 6 and 7 are reduced in size, the nerve terminals synapsed to muscle M12 are greatly reduced and the nerve terminals synapsed to muscle M13 are expanded compared to controls.
Df(3R)Tl-X/Df(3R)ro-XB3 embryos hatch.
Hemocyte density in the hemolymph of Df(3R)Tl-X/Df(3R)ro-XB3 larvae is significantly lower than in the hemolymph of control larvae.
Df(3R)Tl-X/Df(3R)ro-XB3 transheterozygotes are Tl null embryos. RP3 motoneuron axon pathfinding is normal, but the growth cone often misinnervates non-target muscle cells. SNa growth cones develop normally but SNb growth cones lose targeting accuracy. SNb ending at muscle 15/16 cleft appear thicker than normal and innervation at muscle 6/7 cleft is often either missing or reduced in size.
Df(3R)ro-XB3/Df(3R)Tl-X embryos have an altered number of RP neurons. They have ectopically placed motor endings on muscles 6 and 7 and lack normal cleft endings.
In Df(3R)Tl-X/Df(3R)ro-XB3 embryos, 12.1% of hemisegments lack normal innervation of muscle fibers 7 and 6 (though innervation of muscles 15 and 16 is normal), and 29.4% of hemisegments have ectopic endings on muscle fibers 7 and 6 (compared to 0% for muscles 15 and 16). Aberrant numbers of RP motoneurons were detected in 15.5% of hemisegments per embryo, and the positioning of cells is noticeably more irregular than in wild type. The most common phenotype is a hemisegment missing one RP motoneuron in the position of either RP1 or RP4. 86% of mutant animals examined show this phenotype. Embryos and larvae (both Df(3R)Tl-X/Df(3R)ro-XB3 and deficiency heterozygotes) show an average of 46.1% of hemisegments per larva with one or more errors in the muscle pattern. Errors include duplications, missing fibers and misinserted fibers.
Peter Lewis.
Ref: FBrf0059080.
Left limit of break 1 from polytene analysis (FBrf0082809) Right limit of break 1 from inclusion of Tl (FBrf0042050) Limits of break 2 from polytene analysis (FBrf0082809)