FlyBase Recombinant Construct Report: P{UAS-CD4-spGFP11}

UAS regulatory sequences drive expression of the spGFP11 split fluorescent protein fragment fused to a membrane targeting sequence (Tag:M(hCD4)). This construct is designed for use in a GRASP (GFP Reconstitution Across Synaptic Partners) assay, in which complementary split fluorescent protein fragments expressed on the extracellular membrane of different neurons can reconstitute a functional fluorescent protein at points of contact between the neurons.

(Frank et al., 2015)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{spGFP11.UAS.Tag:M(hCD4)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

64315

w^*; P{lexAop-nSyb-spGFP1-10}2, P{UAS-CD4-spGFP11}2; MKRS/TM6B

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

P{UAS-CD4-spGFP11}

(FlyBase, 1992-)

UAS spGFP11::CD4

(Sancer et al., 2019)

UAS-CD4-spGFP-11

(Tenedini et al., 2019)

UAS-CD4-spGFP11

(Qi et al., 2021, Sancer et al., 2019)

UAS-CD4-spGFP₁₁

(Coates et al., 2017)

UAS-CD4:GFP₁₁

(Patel et al., 2022)

UAS-CD4:GFP¹¹

(Du et al., 2018)

UAS-CD4:spGFP11

(Liu et al., 2019, Frank et al., 2015, Macpherson et al., 2015)

UAS.CD4-spGFP11

(Frank et al., 2015)

Secondary FlyBase IDs

References (10)

Report Sections

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