UASt regulatory sequences are fused upstream of the wg 5'UTR and signal sequence, three copies of the Tag:HA tag and the robo2 coding sequence.
Ten copies of the UAS regulatory sequence drive expression of the wild-type robo2 coding sequence which is tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.