FB2024_03 , released June 25, 2024
Allele: Dmel\robo210xUAS.Tag:HA,Tag:SS(wg)
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General Information
Symbol
Dmel\robo210xUAS.Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0316445
Feature type
allele
Associated gene
Dmel\robo2
Associated Insertion(s)
Carried in Construct
M{10xUAS-HA-robo2}
Key Links
Transgenic product class
wild_type
(Evans and Bashaw, 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Evans and Bashaw, 2012)
Mutagen
in vitro construct
(Evans et al., 2015, Evans and Bashaw, 2012)
Progenitor genotype
Carried in construct
M{10xUAS-HA-robo2}
Cytology
Description

Ten copies of the UAS regulatory sequence drive expression of the wild-type robo2 coding sequence which is tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.

(Evans et al., 2015)

UASt regulatory sequences are fused upstream of the wg 5'UTR and signal sequence, three copies of the Tag:HA tag and the robo2 coding sequence.

(Evans and Bashaw, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
UASt
Encoded product / tool
robo2
Tagged with
Tag:HA
Tag:SS(wg)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of robo210xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu results in a biphasic phenotype in the embryonic central nervous system: some segments are almost commissureless, while others show ectopic crossing of the midline by Fas2-positive axons (more than 80% of segments).

      Expression of robo210xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos results in 57.8% of EW neurons failing to cross the midline.

      Expression of robo210xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4sli.PS in embryos results in ectopic crossing of the midline by Fas2-positive axons in almost 80% of segments. 20% of segments have fused commissures.

      (Evans et al., 2015)

      Expression of robo210xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos results in the EW axons failing to cross the midline in just over half of segments.

      (Evans and Bashaw, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      robo210xScer\UAS.T:Ivir\HA1,T:SS-wg
      robo210xUAS.Tag:HA,Tag:SS(wg)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)