FlyBase Recombinant Construct Report: P{6TCF-Helper-lacZ}

General Information

Symbol

P{6TCF-Helper-lacZ}

FlyBase ID

FBtp0095640

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^6TCF-Helper

Also Known As

TCF-LacZ, 6TH-LacZ

Size

Expression Data

lacZ reporter

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Ecol\lacZ^6TCF-Helper

Transgenic product class

Component

Use(s)

Encoded product / tool

lacZ

reporter enzyme

Description

lacZ expression is driven by 6 pan binding sites flanked by 6 synthetic 'Helper' sites (GCCGCCA), and a hsp70 core promoter.

(Chang et al., 2008)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^6TCF-Helper

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

embryonic/larval ostium

(Trujillo et al., 2016)

third instar larval stage

wing margin

(Wang et al., 2013)

wing pouch | surrounding

(Wang et al., 2013)

mesothoracic tergum primordium | restricted

(Wang et al., 2013)

adult stage

muscle cell of proventriculus

(Wang et al., 2013)

adult midgut region R2 -- adult midgut region R3

(Wang et al., 2013)

hindgut boundary cell

(Wang et al., 2013)

adult stage | female

posterior escort cell

(Mottier-Pavie et al., 2016)

Additional Information

Statement

Reference

EcollacZ^6TCF-Helper acts as a reporter for wg activity.

(Wang et al., 2013)

Marker for

embryonic/larval ostium

(Trujillo et al., 2016)

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

68166

w^*; P{6TCF-Helper-lacZ}2

68167

w^*; P{6TCF-Helper-lacZ}3

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

6TH-LacZ

(Mottier-Pavie et al., 2016)

6TH-lacZ

(Chen et al., 2016)

6THlacZ

(Trujillo et al., 2016)

6xTCF-Helper-LacZ

(Erazo-Oliveras et al., 2023)

P{6TCF-Helper-lacZ}

(FlyBase, 1992-)

TCF-LacZ

(Erazo-Oliveras et al., 2023, Wang et al., 2013)

Secondary FlyBase IDs

References (8)

Report Sections

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