phiC31-mediated recombination has been used to replace the phiC31\attP cassette containing the complete 1360 element which is present in the P{w+(FRT.attP.1360)} docking site with sequence (from a donor construct) encoding a partial invader4 element (lacks the long terminal repeats). The invader4 sequence present in the recombinant P{w+(FRT.invader4ΔLTR)(loxP.y+)} element is flanked by Scer\FRT sites. A P1\loxP construct containing a y[+] marker (originating from the donor construct) and a w+mW.hs marker (originating from the docking site) are also present.
phiC31-mediated recombination has been used to replace the phiC31\attP cassette containing the complete 1360 element which is present in the P{w+(FRT.attP.1360)} docking site with sequence (from a donor construct) encoding a partial invader4 element (lacks the long terminal repeats). The invader4 sequence present in the recombinant P{w+(FRT.invader4ΔLTR)(loxP.y+)} element is flanked by Scer\FRT sites. A P1\loxP construct containing a y[+] marker (originating from the donor construct) and a w+mW.hs marker (originating from the docking site) are also present.