FB2024_03 , released June 25, 2024
Recombinant Construct: P{w+(FRT.1360ΔpiRNA)(loxP.y+)}
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General Information
Symbol
P{w+(FRT.1360ΔpiRNA)(loxP.y+)}
FlyBase ID
FBtp0093155
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

phiC31-mediated recombination has been used to replace the phiC31\attP cassette containing the complete 1360 element which is present in the P{w+(FRT.attP.1360)} docking site with sequence (from a donor construct) encoding a partial 1360 element (lacks the left half of the element and lacks the signature ping-pong overlapping peaks (36bp deletion) and the neighbouring shorter antisense peak (10 deletion) of piRNA producing sequence). The partial 1360 sequence present in the recombinant P{w+(FRT.1360ΔpiRNA)(loxP.y+)} element is flanked by Scer\FRT sites. A P1\loxP construct containing a y[+] marker (originating from the donor construct) and a w+mW.hs marker (originating from the docking site) are also present.

(Sentmanat and Elgin, 2012)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{w+(FRT.1360ΔpiRNA)(loxP.y+)}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)