A 2684 base pair fragment from the flanking non-coding or intronic region of nAcRalpha-7E is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Positive GRASP (GFP reconstitution across synaptic partners) signals between lobula plate intrinsic neurons LPi4-3 labelled with EcollexAGMR20D01 and vertical system neurons labelled with ScerGAL4DB331 or ScerGAL4VT058488 were observed.