A 2684 base pair fragment from the flanking non-coding or intronic region of nAcRalpha-7E is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Positive GRASP (GFP reconstitution across synaptic partners) signals between lobula plate intrinsic neurons LPi4-3 labelled with EcollexAGMR20D01 and vertical system neurons labelled with ScerGAL4DB331 or ScerGAL4VT058488 were observed.
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.