FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-NTF}
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General Information
Symbol
P{UAS-NTF}
FlyBase ID
FBtp0071772
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\birAUAS.cSa
RanGAPUAS.Tag:FLAG,Tag:BLRP,mCherry
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele RanGAPUAS.Tag:FLAG,Tag:BLRP,mCherry
Transgenic product class
wild_type
(Steiner et al., 2012)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
RanGAP
Tagged with
mCherry
Tag:BLRP
Tag:FLAG
Description

UAS regulatory sequences drive expression of RanGAP which is tagged at the N-terminal end with three copies of Tag:FLAG, Tag:BLRP and mCherry.

(Steiner et al., 2012)
Component allele Ecol\birAUAS.cSa
Transgenic product class
wild_type
(Steiner et al., 2012)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ecol\birA
Description

UASt regulatory sequences drive expression of Ecol\birA.

(Steiner et al., 2012)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

The P{UAS-NTF} construct allows purification of nuclei from cells via in vivo biotin-labelling of the nuclear envelope followed by affinity purification of the nuclei. The use of tissue-specific Scer\GAL4 drivers to drive expression of P{UAS-NTF} potentially allows nuclei to be purified from a large variety of cell types.

(Steiner et al., 2012)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
P{UAS-NTF}
(FlyBase, 1996-)
RAN-GAP-mcherry-FLAG-BirA
(Ramalingam et al., 2023)
UAS-3xFLAG-blrp-mCherry-RanGap, UAS-BirA
(Ramalingam et al., 2023)
UAS-INTACT
(Domsch et al., 2019)
UAS-NTF
(Steiner et al., 2012)
UAS-RG6
(Jiang et al., 2021)
p4UAS-RG
(Steiner et al., 2012)
Secondary FlyBase IDs
    References (5)