FB2024_03 , released June 25, 2024
Recombinant Construct: P{Gr28b.d-GAL4}
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General Information
Symbol
P{Gr28b.d-GAL4}
FlyBase ID
FBtp0054529
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4Gr28b.d.PT
Also Known As
Gr28b.d-GAL4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4Gr28b.d.PT
Transgenic product class
Component
Use(s)
Regulatory region(s)
Gr28b
Gr28b_Gr28b.d
Encoded product / tool
GAL4
Description

A 772bp fragment containing the promoter region immediately upstream of the d-isoform of Gr28b regulates expression of a GAL4 driver.

(Thorne and Amrein, 2008)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
gustatory receptor neuron of the hypophysis
(Mishra et al., 2018)
gustatory receptor neuron of the epiphysis
(Mishra et al., 2018)
embryonic/larval brain | restricted
(Mishra et al., 2018)
larval ventral nerve cord | restricted
(Mishra et al., 2018)
adult stage
high salt/bitter-sensing neuron | subset of prothoracic tarsal segment 5
(Ling et al., 2014)
gustatory receptor neuron | subset
(Thorne and Amrein, 2008)
gustatory receptor neuron of labellar taste bristle | subset
(Thorne and Amrein, 2008)
gustatory receptor neuron | subset of ventral cibarial sense organ
(Thorne and Amrein, 2008)
gustatory receptor neuron | faint | subset of prothoracic tarsal segment 5
(Thorne and Amrein, 2008)
neuron | faint | subset of prothoracic tarsal segment 5
(Thorne and Amrein, 2008)
neuron | subset of arista
(Thorne and Amrein, 2008)
campaniform sensillum | subset of wing vein L3
(Thorne and Amrein, 2008)
campaniform sensillum | subset of wing vein L1
(Thorne and Amrein, 2008)
mechanosensory neuron | subset of leg
(Thorne and Amrein, 2008)
prothoracic femoral chordotonal organ
(Thorne and Amrein, 2008)
metathoracic femoral chordotonal organ
(Thorne and Amrein, 2008)
mesothoracic femoral chordotonal organ
(Thorne and Amrein, 2008)
dendritic arborizing neuron | subset of abdomen | dorsal
(Thorne and Amrein, 2008)
aristal sensory neuron VP2
(Liu et al., 2015, Ni et al., 2013)
neuron | subset of wing vein L3
(Ni et al., 2013)
neuron | subset of femur
(Ni et al., 2013)
neuron | subset of tarsal segment
(Ni et al., 2013)
neuron | subset of proboscis
(Ni et al., 2013)
gustatory receptor neuron | subset of short labellar taste bristle | subset
(Weiss et al., 2011)
high salt/bitter-sensing neuron of adult labellum sensillum
(Weiss et al., 2011)
short labellar taste bristle S-b

Comment: isoform Gr28b-RD

(Weiss et al., 2011, Weiss et al., 2011)
adult antennal lobe glomerulus
(Thorne and Amrein, 2008)
adult abdominal multidendritic neuron | subset
(Park and Kwon, 2011)
Additional Information
Statement
Reference

ScerGAL4Gr28b.d.PT drives expression in the pattern of the isoform Gr28b-RD.

(Bloomington Drosophila Stock Center, 2017)

ScerGAL4Gr28b.d.PT drives expression in three aristal sensory neuron VP2.

(Liu et al., 2015)

ScerGAL4Gr28b.d.PT drives expression of Gr28b isoform d.

(Ling et al., 2014)

Expression detected in three warmth-responsive sensory neurons in the arista.

(Ni et al., 2013)

ScerGAL4Gr28b.d.PT drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Additionally, expression is driven in multidendritic neurons with ladder-like processes along the dorsal abdominal wall.

(Park and Kwon, 2011)

ScerGAL4Gr28b.d.PT is expressed in a gustatory neuron of one bitter-sensitive labellar taste bristle.

(Weiss et al., 2011)

ScerGAL4Gr28b.d.PT is expressed in one short bitter-sensitive sensilla of the labellum.

(Weiss et al., 2011, Weiss et al., 2011)

Expression of ScerGAL4Gr28b.d.PT is seen in approximately 3 neurons per labial palp. ScerGAL4Gr28b.d.PT is found to be expressed in a single neuron associated with the ventral cibarial sense organ. Expression is found in at least two taste neurons and also in several non-chemosensory neurons associated with the fifth tarsal segment. Expression of ScerGAL4Gr28b.d.PT is seen in three neurons located at the base of the arista, which possibly function as thermo- and/or hygroreceptors. Expression is seen in leg neurons thought to be stretch receptors or proprioreceptors. Expression of ScerGAL4Gr28b.d.PT is seen within axons that innervate either antennal lobe glomerulus VP2 or VP3.

(Thorne and Amrein, 2008)
 
Marker for
Reflects expression of
Gr28b
(Bloomington Drosophila Stock Center, 2017, Liu et al., 2015, Ling et al., 2014, Ni et al., 2013, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011, Thorne and Amrein, 2008)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
58996
P{Gr28b.d-GAL4}E29, w*/FM7c
57620
w*; P{Gr28b.d-GAL4}B27; Dr1/TM3, Sb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (21)