FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-Cdc42.CFP.CBD.YFP}
Open Close
General Information
Symbol
P{UAS-Cdc42.CFP.CBD.YFP}
FlyBase ID
FBtp0052806
Feature type
transgenic_transposable_element
Component Allele(s)
Cdc42A-probe.1.UAS
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Cdc42A-probe.1.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Cdc42
Description

UAS regulatory sequences drive expression of a FRET-based genetically encoded 'activation bioprobe' (A-probe.1) that can be used to monitor activation patterns of Cdc42 in vivo. The bioprobe consists of two fluorophores (CFP and YFP) separated by Cdc42 fused to the Cdc42 binding domain (CBD) of WASp. When Cdc42 protein is activated, it binds to the WASp CBD, resulting in a change in protein conformation that allows FRET (fluorescence resonance energy transfer) to occur between the donor (CFP) and acceptor (YFP) fluorescent proteins.

(Kamiyama and Chiba, 2009)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
A-probe.1
(Kamiyama and Chiba, 2009)
P{UAS-Cdc42.CFP.CBD.YFP}
(FlyBase, 1992-)
UAS-YFP::CBD::cdc42::CFP
(Kamiyama et al., 2015)
Secondary FlyBase IDs
    References (3)