FB2024_03 , released June 25, 2024
Allele: Dmel\Cdc42A-probe.1.UAS
Open Close
General Information
Symbol
Dmel\Cdc42A-probe.1.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0242692
Feature type
allele
Associated gene
Dmel\Cdc42
Associated Insertion(s)
Carried in Construct
P{UAS-Cdc42.CFP.CBD.YFP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kamiyama and Chiba, 2009)
Progenitor genotype
Carried in construct
P{UAS-Cdc42.CFP.CBD.YFP}
Cytology
Description

UAS regulatory sequences drive expression of a FRET-based genetically encoded 'activation bioprobe' (A-probe.1) that can be used to monitor activation patterns of Cdc42 in vivo. The bioprobe consists of two fluorophores (CFP and YFP) separated by Cdc42 fused to the Cdc42 binding domain (CBD) of WASp. When Cdc42 protein is activated, it binds to the WASp CBD, resulting in a change in protein conformation that allows FRET (fluorescence resonance energy transfer) to occur between the donor (CFP) and acceptor (YFP) fluorescent proteins.

(Kamiyama and Chiba, 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Cdc42
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cdc42A-probe.1.UAS
      Cdc42Scer\UAS.T:Avic\GFP-CFP,T:CBD,T:Avic\GFP-YFP
      Cdc42Scer\UAS.T:Avic\GFP-CFP,T:CBD-WASp,T:Avic\GFP-YFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)