FB2024_03 , released June 25, 2024
Recombinant Construct: P{PCNA-Emerald}
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General Information
Symbol
P{PCNA-Emerald}
FlyBase ID
FBtp0039306
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPPCNA.Emerald
Also Known As
PCNA-EmGFP
Size
Expression Data
GFP reporter
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPPCNA.Emerald
Transgenic product class
Component
Use(s)
Regulatory region(s)
PCNA
Encoded product / tool
Emerald
Description

Expression of three copies of the Emerald fluorescent protein is driven by an approximately 100bp region from immediately upstream of the PCNA coding region, containing two E2F binding sites.

(Swanhart et al., 2007)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
late third instar larval stage
neuroblast
(Siegrist et al., 2010)
pupal stage P5(ii)
neuroblast
(Siegrist et al., 2010)
pharate adult stage P8
neuroblast
(Siegrist et al., 2010)
Additional Information
Statement
Reference

Reporter construct responsive to E2f.

(Siegrist et al., 2010)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
24904
w1; P{PCNA-Emerald}T137
25677
P{PCNA-Emerald}T136, w*
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
PCNA-EmGFP
(Dichtel-Danjoy et al., 2013, Kawamori and Yamaguchi, 2011, Swanhart et al., 2007)
PCNA::3XEmGFP
(Kuang et al., 2014)
P{PCNA-EmGFP}
(FlyBase, 1996-)
P{PCNA-Emerald}
(FlyBase, 1996-)
P{mus209-EmGFP}
(Kawamori and Yamaguchi, 2011, Duronio, 2008.10.22, FlyBase, 1992-)
pcna-3XEmgfp
(Weng et al., 2012)
pcna-GFP
(Moraru et al., 2012)
pcna:eGFP
(Siegrist et al., 2010)
Secondary FlyBase IDs
    References (11)