iav is expressed from its own promoter and tagged with EGFP at the C-terminus. The EGFP tag is inserted into the HindIII site in exon 7 of iav.
iavCterm.T:AvicGFP-EGFP is expressed in the scolopidia of the Johnston's organ and is concentrated in the proximal tip of the cilia in each scolopidial extracellular space.