Transgenic construct used to analyze the role of the Trl (GAGA) protein in the enhancer-blocking activity of Fab-7, a domain boundary element from the bithorax complex. Derived from P{wRW+we}; a 1.2kb XhoI-NotI fragment corresponding to the minimal Fab-7 boundary element has been inserted between the w enhancer and w transcription unit.
Consists of a 1231bp PstI-ApaI fragment including the Fab-7 insulator.
A 1.2kb XhoI-NotI fragment corresponding to the minimal Fab-7 boundary element.
Construct: 1.2kb Fab-7 DNA fragment that spans nucleotide positions 84,594 to 83,423 within the complete BX-C sequence and contains all three DNaseI hypersensitive sites.
Construct: 0.5kb of scs' sequences.